Home / WNK1 / Human Phospho-WNK1 (T60) Antibody

Human Phospho-WNK1 (T60) Antibody

Catalog #: MAB4720
1 Citation Datasheet / COA / SDS
Recombinant Monoclonal Antibody
Catalog # Availability Size / Price Qty
MAB4720-SP
MAB4720-100
Detection of Human Phospho-WNK1 (T60) by Western Blot.
1 Image
Product Details
Citations (1)
FAQs
Supplemental Products
Reviews
Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Phospho-WNK1 (T60) Antibody Summary

Species Reactivity
Human
Specificity
Detects human WNK1 when phosphorylated at T60 in Western blots.
Source
Recombinant Monoclonal Rabbit IgG Clone # 1259F
Purification
Protein A or G purified from cell culture supernatant
Immunogen
Phosphopeptide containing human WNK1 T60 site
Accession # Q9H4A3
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human Phospho-WNK1 (T60) antibody by Western Blot. View Larger

Detection of Human Phospho-WNK1 (T60) by Western Blot. Western blot shows lysates of HEK293 human embryonic kidney cell line untreated (-) or treated (+) with 1 µg/mL insulin for 5 minutes. PVDF membrane was probed with 1 µg/mL of Rabbit Anti-Human Phospho-WNK1 (T60) Monoclonal Antibody (Catalog # MAB4720) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for Phospho-WNK1 (T60) at approximately 280 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Loading...
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: WNK1

With No Lysine (K) 1 (WNK1) belongs to a novel family of serine/threonine protein kinases involved in regulating ion transport. The WNKs contain a cysteine instead of the usual lysine in their kinase activation domains. WNK1 mutations have been implicated in Pseudohypoaldosteronism type II (PHAII), an autosomal dominant disorder characterized by hypertension, increased renal salt absorption, and impaired potassium and hydrogen excretion. WNK1 is phosphorylated by Akt1 and Akt2 at T60, and may function as a negative regulator of insulin-stimulated mitogenesis.

Long Name
WNK Lysine-deficient Protein Kinase 1
Entrez Gene IDs
65125 (Human); 232341 (Mouse); 116477 (Rat)
Alternate Names
EC 2.7.11; EC 2.7.11.1; Erythrocyte 65 kDa protein; HSAN2hereditary sensory neuropathy, type II; HSN2MGC163339; hWNK1; KDP; KDPMGC163341; KIAA0344; Kinase deficient protein; p65; PHA2C; PRKWNK1; PRKWNK1PSK; prostate-derived sterile 20-like kinase; Protein kinase lysine-deficient 1; Protein kinase with no lysine 1; protein kinase, lysine deficient 1; serine/threonine-protein kinase WNK1; WNK lysine deficient protein kinase 1; WNK1

Product Datasheets

You must select a language.

x
Product Datasheet
COA
SDS

Citation for Human Phospho-WNK1 (T60) Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Soluble bone-derived osteopontin promotes migration and stem-like behavior of breast cancer cells
    Authors: GM Pio, Y Xia, MM Piaseczny, JE Chu, AL Allan
    PLoS ONE, 2017-05-12;12(5):e0177640.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot

FAQs

No product specific FAQs exist for this product, however you may

View all Antibody FAQs
Loading...

Reviews for Human Phospho-WNK1 (T60) Antibody

There are currently no reviews for this product. Be the first to review Human Phospho-WNK1 (T60) Antibody and earn rewards!

Have you used Human Phospho-WNK1 (T60) Antibody?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥2500 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review