Home / CXCL16 / Mouse CXCL16 Antibody

Mouse CXCL16 Antibody

Catalog #: MAB503
15 Citations 1 Review Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB503-SP
MAB503-500
MAB503-100
Chemotaxis Induced by CXCL16 and Neutralization by Mouse CXCL16 Antibody.
1 Image
Product Details
Citations (15)
FAQs
Supplemental Products
Reviews (1)
Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse CXCL16 Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse CXCL16 in ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human CXCL1, 2, 3, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, recombinant mouse CXCL1, 2, 6, 9, 10, 11, 12, 13, 14, 15, recombinant rat CXCL1, 2, 3, 5, or recombinant porcine CXCL8 is observed.
Source
Monoclonal Rat IgG2A Clone # 142417
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant mouse CXCL16
Asn27-Pro114
Accession # Q8BSU2
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
Recombinant Mouse CXCL16 Chemokine Domain (Catalog # 503-CX) under non-reducing conditions only

Mouse CXCL16 Sandwich Immunoassay

Recommended Concentration
Reagent
ELISA Capture (Matched Antibody Pair)
2-8 µg/mL 

Use in combination with:

Detection Reagent: Mouse CXCL16 Biotinylated Antibody (Catalog # BAF503)

Standard: Recombinant Mouse CXCL16 Protein (Catalog # 503-CX)

Neutralization
Measured by its ability to neutralize CXCL16-induced chemotaxis in the BaF3 mouse pro‑B cell line transfected with mouse CXCR6. Matloubian, M. et al. (2000) Nature Immunol. 1:298. The Neutralization Dose (ND50) is typically 0.15-0.6 µg/mL in the presence of 7.5 ng/mL Recombinant Mouse CXCL16 Chemokine Domain.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Neutralization Chemotaxis Induced by CXCL16 and Neutralization by Mouse CXCL16 Antibody. View Larger

Chemotaxis Induced by CXCL16 and Neutralization by Mouse CXCL16 Antibody. Recombinant Mouse CXCL16 Chemokine Domain (Catalog # 503-CX) chemoattracts the BaF3 mouse pro-B cell line transfected with mouse CXCR6 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Mouse CXCL16 Chemokine Domain (7.5 ng/mL) is neutralized (green line) by increasing concentrations of Rat Anti-Mouse CXCL16 Monoclonal Antibody (Catalog # MAB503). The ND50 is typically 0.15-0.6 µg/mL.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Loading...
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CXCL16

Mouse CXCL16 (CXC chemokine 16) is a non-ELR motif-containing CXC chemokine with a transmembrane domain. CX3CL1/Fractalkine andCXCL16 are the only two transmembrane chemokines within the superfamily. Mouse CXCL16 cDNA encodes a 246 amino acid (aa) precursor protein with a putative 26 aa signal peptide, an 88 aa chemokine domain, an 87 aa mucin-like spacer region, a 22 aa transmembrane domain, and a 23 aa cytoplasmic tail. Mouse and human CXCL16 share 49% aa sequence identity. Mouse CXCL16 is produced by dendritic cells in lymphoid organ T cell zones and bycells in the splenic red pulp both as membrane-bound and soluble forms. Based on northern blot analysis, CXCL16 is also expressed in some non-lymphoid tissues such as lung, small intestine and kidney. The receptor for CXCL16 has been identified as CXCR6 (previously called Bonzo, STRL33 or TYMSTR), which is also a coreceptor for HIV entry. CXCR6 is expressed on naive CD8 cells, natural killer T cells and activated CD8 and CD4 T cells.

Entrez Gene IDs
58191 (Human); 66102 (Mouse)
Alternate Names
chemokine (C-X-C motif) ligand 16; CXC chemokine ligand 16; CXCL16; CXCLG16; Scavenger receptor for phosphatidylserine and oxidized low density lipoprotein; SCYB16; Small-inducible cytokine B16; SRPSOXC-X-C motif chemokine 16; SR-PSOXTransmembrane chemokine CXCL16

Product Datasheets

You must select a language.

x
Product Datasheet
COA
SDS

Citations for Mouse CXCL16 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

15 Citations: Showing 1 - 10
Filter your results:

Filter by:

  1. Single-cell RNA transcriptome analysis of CNS immune cells reveals CXCL16/CXCR6 as maintenance factors for tissue-resident T cells that drive synapse elimination
    Authors: Sarah F. Rosen, Allison L. Soung, Wei Yang, Shenjian Ai, Marlene Kanmogne, Veronica A. Davé et al.
    Genome Medicine
  2. Oncogenic collagen I homotrimers from cancer cells bind to alpha 3 beta 1 integrin and impact tumor microbiome and immunity to promote pancreatic cancer
    Authors: Chen Y, Yang S, Tavormina J et al.
    Cancer cell
  3. Innate acting memory Th1 cells modulate heterologous diseases
    Authors: Rakebrandt, N;Yassini, N;Kolz, A;Schorer, M;Lambert, K;Goljat, E;Estrada Brull, A;Rauld, C;Balazs, Z;Krauthammer, M;Carballido, JM;Peters, A;Joller, N;
    Proceedings of the National Academy of Sciences of the United States of America
    Species: Mouse
    Sample Types: In Vivo
    Applications: Neutralization
  4. Sepsis-trained macrophages promote antitumoral tissue-resident T cells
    Authors: Broquet, A;Gourain, V;Goronflot, T;Le Mabecque, V;Sinha, D;Ashayeripanah, M;Jacqueline, C;Martin, P;Davieau, M;Boutin, L;Poulain, C;Martin, FP;Fourgeux, C;Petrier, M;Cannevet, M;Leclercq, T;Guillonneau, M;Chaumette, T;Laurent, T;Harly, C;Scotet, E;Legentil, L;Ferrières, V;Corgnac, S;Mami-Chouaib, F;Mosnier, JF;Mauduit, N;McWilliam, HEG;Villadangos, JA;Gourraud, PA;Asehnoune, K;Poschmann, J;Roquilly, A;
    Nature immunology
    Species: Mouse
    Sample Types: In Vivo
    Applications: In vivo assay
  5. LSD1 drives intestinal epithelial maturation and controls small intestinal immune cell composition independent of microbiota in a murine model
    Authors: Díez-Sánchez, A;Lindholm, HT;Vornewald, PM;Ostrop, J;Yao, R;Single, AB;Marstad, A;Parmar, N;Shaw, TN;Martín-Alonso, M;Oudhoff, MJ;
    Nature communications
    Species: Transgenic Mouse
    Sample Types: In Vivo
    Applications: Neutralization
  6. Mucosal-associated invariant T cells contribute to suppression of inflammatory myeloid cells in immune-mediated kidney disease
    Authors: Gnirck, AC;Philipp, MS;Waterhölter, A;Wunderlich, M;Shaikh, N;Adamiak, V;Henneken, L;Kautz, T;Xiong, T;Klaus, D;Tomczyk, P;Al-Bahra, MM;Menche, D;Walkenhorst, M;Lantz, O;Willing, A;Friese, MA;Huber, TB;Krebs, CF;Panzer, U;Kurts, C;Turner, JE;
    Nature communications
    Species: Mouse
    Sample Types: In Vivo
    Applications: In Vivo
  7. Targeting CXCL16 and STAT1 augments immune checkpoint blockade therapy in triple-negative breast cancer
    Authors: B Palakurthi, SR Fross, IH Guldner, E Aleksandro, X Liu, AK Martino, Q Wang, RA Neff, SM Golomb, C Lewis, Y Peng, EN Howe, S Zhang
    Nature Communications, 2023-04-13;14(1):2109.
    Species: Mouse
    Sample Types: Whole Tissues
    Applications: IHC
  8. Targeting cardiomyocyte ADAM10 ectodomain shedding promotes survival early after myocardial infarction
    Authors: E Klapproth, A Witt, P Klose, J Wiedemann, N Vavilthota, SR Künzel, S Kämmerer, M Günscht, D Sprott, M Lesche, F Rost, A Dahl, E Rauch, L Kattner, S Weber, P Mirtschink, I Kopaliani, K Guan, K Lorenz, P Saftig, M Wagner, A El-Armouch
    Nature Communications, 2022-12-10;13(1):7648.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  9. Inflammation triggers ILC3 patrolling of the intestinal barrier
    Authors: A Jarade, Z Garcia, S Marie, A Demera, I Prinz, P Bousso, JP Di Santo, N Serafini
    Nature Immunology, 2022-08-23;0(0):.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In Vivo
  10. Phosphodiesterase 5 (PDE5) Is Highly Expressed in Cancer-Associated Fibroblasts and Enhances Breast Tumor Progression
    Authors: S Catalano, S Panza, G Augimeri, C Giordano, R Malivindi, L Gelsomino, S Marsico, F Giordano, B Gy?rffy, D Bonofiglio, S Andò, I Barone
    Cancers (Basel), 2019-11-06;11(11):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Neutralization
  11. Response patterns of cytokines/chemokines in two murine strains after irradiation.
    Authors: Zhang M, Yin L, Zhang K, Sun W, Yang S, Zhang B, Salzman P, Wang W, Liu C, Vidyasagar S, Zhang L, Ju S, Okunieff P, Zhang L
    Cytokine, 2012-01-25;58(2):169-77.
    Species: Mouse
    Sample Types: Plasma
    Applications: Luminex Development
  12. Excreted urinary mediators in an animal model of experimental immune nephritis with potential pathogenic significance.
    Authors: Wu T, Xie C, Bhaskarabhatla M, Yan M, Leone A, Chen SS, Zhou XJ, Putterman C, Mohan C
    Arthritis Rheum., 2007-03-01;56(3):949-59.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Neutralization
  13. A disintegrin and metalloproteinase 10-mediated cleavage and shedding regulates the cell surface expression of CXC chemokine ligand 16.
    Authors: Gough PJ, Garton KJ, Wille PT, Rychlewski M, Dempsey PJ, Raines EW
    J. Immunol., 2004-03-15;172(6):3678-85.
    Species: Mouse
    Sample Types: Cell Culture Supernates, Whole Cells
    Applications: ELISA Development, Flow Cytometry
  14. CXCL16/CXCR6 Axis Drives Microglia/Macrophages Phenotype in Physiological Conditions and Plays a Crucial Role in Glioma
    Authors: Lepore F, D'Alessandro G, Antonangeli F et al.
    Front Immunol
  15. Role of MR1-driven signals and amphiregulin on the recruitment and repair function of MAIT cells during skin wound healing
    Authors: Anastasia du Halgouet, Aurélie Darbois, Mansour Alkobtawi, Martin Mestdagh, Aurélia Alphonse, Virginie Premel et al.
    Immunity

FAQs

No product specific FAQs exist for this product, however you may

View all Antibody FAQs
Loading...

Reviews for Mouse CXCL16 Antibody

Average Rating: 5 (Based on 1 Review)

5 Star
100%
4 Star
0%
3 Star
0%
2 Star
0%
1 Star
0%

Have you used Mouse CXCL16 Antibody?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥2500 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review

Filter by:


Mouse CXCL16 Antibody
By Anonymous on 09/24/2021
Application: WB Sample Tested: BaF3 mouse pro‑B cell line Species: Mouse
Western Blot Mouse CXCL16 Antibody MAB503