Mouse PlGF-2 Antibody

Catalog #: MAB465R Datasheet / COA / SDS

Recombinant Monoclonal Antibody

Catalog #	Availability	Size / Price	Qty
MAB465R-100
MAB465R-SP

All PlGF-2 Antibodies

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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse PlGF-2 Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse PIGF-2 in direct ELISAs.

Source

Recombinant Monoclonal Rat IgG_2A Clone # 62526R

Purification

Protein A or G purified from cell culture supernatant

Immunogen

S. frugiperda insect ovarian cell line Sf 21-derived recombinant mouse PlGF-2
Ala24-Pro158
Accession # P49764

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Blockade of Receptor-ligand Interaction

In a functional ELISA, 0.015-0.06 μg/mL of this antibody will block 50% of the binding of 4 ng/mL of Recombinant Mouse PlGF-2 to immobilized Recombinant Human Flt-1/Fc Chimera coated at 1 μg/mL (100 µL/well).

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: PlGF-2

PlGF is a member of the VEGF family of growth factors, and it binds with high-affinity to VEGF R1. As a result of alternative splicing, three human PlGF mRNAs encode PlGF-1 (PlGF₁₃₁), PlGF-2 (PlGF₁₅₂) and PlGF-3 (PlGF₂₀₃). Normal mouse tissues express only a 158 amino acid precursor that is the equivalent of human PlGF-2.

Long Name

Placenta Growth Factor 2

Entrez Gene IDs

5228 (Human); 18654 (Mouse)

Alternate Names

OORS; PlGF2; PlGF-2