Key Product Details

Species Reactivity

Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all Testisin/Prss21 Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse Testisin/Prss21
Gln28-Asn298
Accession # Q9JHJ7

Specificity

Detects recombinant mouse Testisin/Prss21 in direct ELISAs and Western blots. Detects mouse and rat Testisin/Prss21 in Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant mouse (rm) Prss8 and rmPrss27 is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Mouse/Rat Testisin/Prss21 Antibody

Detection of Mouse and Rat Testisin/Prss21 antibody by Western Blot.

Detection of Mouse and Rat Testisin/Prss21 by Western Blot.

Western blot shows lysates of mouse testis tissue and rat testis tissue. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Mouse Testisin/Prss21 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6820) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Testisin/Prss21 at approximately 40 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Testisin/Prss21 antibody in Mouse Testis by Immunohistochemistry (IHC-Fr).

Testisin/Prss21 in Mouse Testis.

Testisin/Prss21 was detected in perfusion fixed frozen sections of mouse testis using Goat Anti-Mouse Testisin/Prss21 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6820) at 1.7 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to the plasma membranes of spermatocytes. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections. This application has not been tested in rat samples.

Applications for Mouse/Rat Testisin/Prss21 Antibody

Application
Recommended Usage

Immunohistochemistry

5-15 µg/mL
Sample: Perfusion fixed frozen sections of mouse testis

Western Blot

0.5 µg/mL
Sample: Mouse testis tissue and rat testis tissue

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Sterile PBS to a final concentration of 0.2 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Testisin/Prss21

Testisin is a serine protease encoded by the PRSS21 gene. Testisin is similar in structure to tryptases, and is also known as Tryptase‑4 (1). It is predicted to have substrate specificity similar to that of trypsin (2). Testisin is most highly expressed in testicular germ cells, but is also present in eosinophils (3). Testisin is known to be required for spermatogenesis, and deficiencies of the enzyme can result in infertility (4, 5). Testisin is anchored to the cell surface through a C-terminal glycosylphosphatidylinositol anchor (6). Recombinant mouse Testisin is expressed with a truncated C-terminus, resulting in its secretion. The recombinant protein was purified as the zymogen and can undergo autoactivation under the appropriate conditions. Over aa 28-298, mouse Testisin shares 92% aa identity with rat Testisin.

References

  1. Wong, G.W. et al. (2001) J. Biol. Chem. 276:20648.
  2. Hooper, J.D. et al. (2000) Biochim. Biophys. Acta 1492:63.
  3. Inoue, M. et al. (1998) Biochem. Biophys.  Res. Comm. 252:307.
  4. Netzel-Arnett, S. et al. (2009) Biol. Reprod. 81:921.
  5. Kawano, N. et al. (2010) Biol. Reprod. 83:359.
  6. Honda, A. et al. (2002) J. Biol. Chem. 277:16976.

Alternate Names

Prss21, TEST1

Entrez Gene IDs

10942 (Human); 57256 (Mouse)

Gene Symbol

PRSS21

UniProt

Q9JHJ7

Additional Testisin/Prss21 Products

Product Documents for Mouse/Rat Testisin/Prss21 Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Mouse/Rat Testisin/Prss21 Antibody

For research use only

Related Research Areas

Serine Proteases and Regulators

Citations for Mouse/Rat Testisin/Prss21 Antibody

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Protocols

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