Mouse/Rat Testisin/Prss21 Antibody

Catalog #: AF6820 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF6820
AF6820-SP

Detection of Mouse and Rat Testisin/Prss21 by Western Blot.

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse/Rat Testisin/Prss21 Antibody Summary

Species Reactivity

Mouse, Rat

Specificity

Detects recombinant mouse Testisin/Prss21 in direct ELISAs and Western blots. Detects mouse and rat Testisin/Prss21 in Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant mouse (rm) Prss8 and rmPrss27 is observed.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse Testisin/Prss21
Gln28-Asn298
Accession # Q9JHJ7

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

0.5 µg/mL

See below

Immunohistochemistry

5-15 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Mouse and Rat Testisin/Prss21 by Western Blot. Western blot shows lysates of mouse testis tissue and rat testis tissue. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Mouse Testisin/Prss21 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6820) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Testisin/Prss21 at approximately 40 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunohistochemistry

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Testisin/Prss21 in Mouse Testis. Testisin/Prss21 was detected in perfusion fixed frozen sections of mouse testis using Goat Anti-Mouse Testisin/Prss21 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6820) at 1.7 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to the plasma membranes of spermatocytes. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections. This application has not been tested in rat samples.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Sterile PBS to a final concentration of 0.2 mg/mL.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Testisin/Prss21

Testisin is a serine protease encoded by the PRSS21 gene. Testisin is similar in structure to tryptases, and is also known as Tryptase‑4 (1). It is predicted to have substrate specificity similar to that of trypsin (2). Testisin is most highly expressed in testicular germ cells, but is also present in eosinophils (3). Testisin is known to be required for spermatogenesis, and deficiencies of the enzyme can result in infertility (4, 5). Testisin is anchored to the cell surface through a C-terminal glycosylphosphatidylinositol anchor (6). Recombinant mouse Testisin is expressed with a truncated C-terminus, resulting in its secretion. The recombinant protein was purified as the zymogen and can undergo autoactivation under the appropriate conditions. Over aa 28-298, mouse Testisin shares 92% aa identity with rat Testisin.

References

Wong, G.W. et al. (2001) J. Biol. Chem. 276:20648.
Hooper, J.D. et al. (2000) Biochim. Biophys. Acta 1492:63.
Inoue, M. et al. (1998) Biochem. Biophys. Res. Comm. 252:307.
Netzel-Arnett, S. et al. (2009) Biol. Reprod. 81:921.
Kawano, N. et al. (2010) Biol. Reprod. 83:359.
Honda, A. et al. (2002) J. Biol. Chem. 277:16976.

Entrez Gene IDs

10942 (Human); 57256 (Mouse)

Alternate Names

EC 3.4.21; Eosinophil serine protease 1; ESP1EC 3.4.21.-; ESP-1serine protease from eosinophils; protease, serine, 21 (testisin); Prss21; Serine protease 21; TEST1; TEST1testisin; Testisin