Mouse Semaphorin 4C Antibody

Catalog #: MAB8497 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB8497
MAB8497-SP

Detection of Semaphorin 4C in HEK293 Human Cell Line Transfected with Mouse Semaphorin 4C and eGFP by Flow Cytometry.

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Mouse Semaphorin 4C Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse Semaphorin 4C in direct ELISAs. Detects HEK293 human embryonic kidney cell line transfected with mouse Semaphorin 4C by Flow Cytometry. Does not detect untransfected or irrelevant transfected HEK293 cells.

Source

Monoclonal Rat IgG₁ Clone # 663203

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse Semaphorin 4C
Ala21-Phe664
Accession # Q64151

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Flow Cytometry

0.25 µg/10⁶ cells

See below

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

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Detection of Semaphorin 4C in HEK293 Human Cell Line Transfected with Mouse Semaphorin 4C and eGFP by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with (A) mouse Semaphorin 4C or (B) irrelevant transfectants and eGFP was stained with Rat Anti-Mouse Semaphorin 4C Monoclonal Antibody (Catalog # MAB8497) followed by Allophycocyanin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0113). Quadrant markers were set based on control antibody staining (Catalog # MAB005).

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Semaphorin 4C

Sema4C (Semaphorin 4C; also Sema I and M-SemaF) is a 100-105 kDa, class IV member of the semaphorin family of proteins. It is expressed by precursors to neurons and myocytes, and may regulate their differentiation into mature forms. Mature mouse Sema4C is a type I transmembrane glycoprotein that is 814 amino acids (aa) in length. It contains a 644 aa extracellular region (aa 21-664) that is characterized by the presence of one Sema domain (aa 30-497), a PSI region (aa 499‑552), and an Ig-like C2-type domain (aa 557-645). The cytoplasmic region interacts with PZD-domain containing proteins. Over aa 21-664, mouse Sema4C shares 85% and 95% aa identity with human and rat Sema4C, respectively.

Entrez Gene IDs

54910 (Human); 20353 (Mouse); 301346 (Rat)

Alternate Names

FLJ20369; KIAA1739; MGC126382; MGC126383; M-Sema F; M-SEMA-F; sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and shortcytoplasmic domain, (semaphorin) 4C; SEMA4C; SEMACL1; Semaf; SEMAI; Semaphorin 4C; semaphorin-4C