Mouse Semaphorin 6A Antibody

Name: Mouse Semaphorin 6A Antibody
Brand: R&D Systems
SKU: MAB1615
Rating: 5 (1 reviews)

Catalog #: MAB1615
2 Citations 1 Review Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB1615
MAB1615-SP

All Semaphorin 6A Antibodies

Product Details

Citations (2)

FAQs

Supplemental Products

Reviews (1)

Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse Semaphorin 6A Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse Semaphorin 6A in direct ELISAs and Western blots. In direct ELISAs, approximately 40% cross-reactivity with recombinant human (rh) Semaphorin 6A is observed and no cross-reactivity with recombinant mouse Semaphorins 3C, 3E, 3F or 7A, or rhSemaphorins 3A, or 3B is observed.

Source

Monoclonal Rat IgG_2A Clone # 201932

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

S. frugiperda insect ovarian cell line Sf 21-derived recombinant mouse Semaphorin 6A
Gly19-Thr649
Accession # O35464

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

Recombinant Mouse Semaphorin 6A

Immunohistochemistry

8-25 µg/mL

Perfusion fixed frozen sections of mouse brain (medulla) and mouse embryo

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Semaphorin 6A

Semaphorin 6A (Sema6A) is a member of the class 6 semaphorin family of type I transmembrane proteins important in axon guidance during neuronal development. Class 6 semaphorins mediate bidirectional signaling, in part through interaction with plexin A1. In addition to neuronal expression, Semaphorin 6A is induced by IFN-gamma in Langerhans cells. Mature mouse and human Semaphorin 6A extracellular domains share 95% amino acid sequence identity.

Entrez Gene IDs

57556 (Human); 20358 (Mouse)

Alternate Names

KIAA1368HT018; sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6A; Sema VIA; SEMA; Sema6A; SEMA6A1; SEMA6A-1; Semaphorin 6A; semaphorin 6A-1; Semaphorin VIA; semaphorin-6A; semaphorin-6A-1; SEMAQ; VIA

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citations for Mouse Semaphorin 6A Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
Filter your results:

Filter by:

Plexin-A2 and its ligand, Sema6A, control nucleus-centrosome coupling in migrating granule cells.
Authors: Renaud J, Kerjan G, Sumita I, Zagar Y, Georget V, Kim D, Fouquet C, Suda K, Sanbo M, Suto F, Ackerman SL, Mitchell KJ, Fujisawa H, Chedotal A
Nat. Neurosci., 2008-03-09;11(4):440-9.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-Fr
Plexin-B2 controls the development of cerebellar granule cells.
Authors: Friedel RH, Kerjan G, Rayburn H, Schuller U, Sotelo C, Tessier-Lavigne M, Chedotal A
J. Neurosci., 2007-04-04;27(14):3921-32.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC