Mouse Semaphorin 6B Antibody

Catalog #: MAB2264 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB2264
MAB2264-SP

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Mouse Semaphorin 6B Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse Semaphorin 6B in direct ELISAs and Western blots. In direct ELISAs, approximately 40% cross‑reactivity with recombinant human (rh) Semaphorin 6B is observed and no cross-reactivity with rhSemaphorin 3A, recombinant mouse Semaphorin 3B, 3C, 3E, 3F, 6A, 6C, 6D, or 7A is observed.

Source

Monoclonal Rat IgG₁ Clone # 256537

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse Semaphorin 6B
Leu27-Ser605 (predicted)
Accession # O54951

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

Recombinant Mouse Semaphorin 6B

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Reconstitution Buffer Available

Reconstitution Buffer 1 (PBS)

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Semaphorin 6B

Semaphorin 6B is a type I transmembrane protein belonging to the Semaphorin family. It is highly expressed in human brain and is expressed at lower levels in other tissues. A splice variant of Semaphorin 6B encodes a secreted protein.

Entrez Gene IDs

10501 (Human); 20359 (Mouse)

Alternate Names

sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6B; Sema6B; SEMAN; Semaphorin 6B; semaphorin VIB; semaphorin Z; semaphorin-6B; semaphorin-6Ba; SEMA-VIB; SEMAZ; SEM-SEMA-Y; UNQ1907/PRO4353