Human GATA-3 Antibody

Detection of Human and Mouse GATA‑3 by Western Blot. Western blot shows lysates of MCF-7 human breast cancer cell line, Jurkat human acute T cell leukemia cell line, and EL-4 mouse lymphoblast cell line. PVDF membrane was probed with 0.25 µg/mL of Goat Anti-Human GATA-3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2605) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). Specific bands were detected for GATA-3 full length (FL) at approximately 55 kDa and the splice form (SF) at approximately 40 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

GATA‑3 in Human Breast Cancer Tissue. GATA-3 was detected in immersion fixed paraffin-embedded sections of human breast cancer tissue using Goat Anti-Human GATA-3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2605) at 3 µg/mL overnight at 4 °C. Before incubation with the primary antibody tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

GATA‑3 in MCF-7 Human Cell Line. GATA-3 was detected in immersion fixed MCF-7 human breast cancer cell line using 10 µg/mL Goat Anti-Human GATA-3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2605) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red, upper panel; Catalog # NL001) and counterstained with DAPI (blue, lower panel). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

GATA‑3 in Human Breast Cancer Tissue. GATA-3 was detected in immersion fixed paraffin-embedded sections of human breast cancer tissue using Goat Anti-Human GATA-3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2605) at 3 µg/mL overnight at 4 °C. Before incubation with the primary antibody tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Detection of Human GATA‑3 by Simple Western^TM. Simple Western lane view shows lysates of MCF-7 human breast cancer cell line and Jurkat human acute T cell leukemia cell line, loaded at 0.2 mg/mL. Specific bands were detected for GATA-3 full length (FL) at approximately 62 kDa and the splice form (SF) at approximately 52 kDa (as indicated) using 2.5 µg/mL of Goat Anti-Human GATA-3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2605) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Detection of Mouse GATA-3 by Immunohistochemistry Generation of a self‐organizing de novo nephron from the differentiated Sca1+ Oct4+ monolayer cultures. A) Bright field image of self‐organizing organoid‐like structure from monolayer culture of kidney stem cells. The photo shows glomerulus‐like structure (black arrow), tubule‐like structure (red arrows), and collecting duct‐like structure (green arrow). Scale bar, 100 µm. A1) Glomerulus‐like structure stained with nephrin (white arrow). Scale bar, 50 µm. A2) Glomerulus‐like structure stained with nephrin (white arrow) and proximal tubule‐like structure stained with LTL (red arrows). Scale bar, 50 µm. A3) Glomerulus‐like structure stained with podocin (white arrow). Scale bar, 50 µm. A4) Collecting duct‐like structure stained with GATA3 (white arrow). Scale bar, 50 µm.,C) Western blot analysis of CD31, nephrin, and podocin expression in undifferentiated and differentiated Sca1+ Oct4+ cells. GAPDH was used as a loading control. Data is representative of three independent experiments. Values represent the mean ± SD (n = 3). p < 0.05 versus control by 2‐tailed Student's t test. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/35315252), licensed under a CC-BY license. Not internally tested by R&D Systems.