Isolation and Culture of Primary Cells from Bone Marrow

Paul Maye, Ph.D., Assistant Professor, University of Connecticut Health Center

Bone marrow stromal cells have been one of the more intensively studied adult stem cell types over the past 50 years. There is great interest within regenerative medicine and wound healing for this adult stem cell type because of its complex biological properties including its ability to support a hematopoietic environment, possess anti-inflammatory qualities, and differentiate down multiple lineages. This webinar discusses research that helps us understand their origin, identity, and biological complexity. This includes:

• Ways to isolate and culture stromal cells.
• in vitro assays of multipotency.
• Assays to quantify stromal cells.
• Benefits of murine transgenic animal models to study stromal cells and their differentiated progeny.

Recorded 12th April 2016