Antibodies for SARS-CoV-2 Immunoassay Development

Antibodies for SARS-CoV-2 Immunoassay Development

Containing the spread of COVID-19 requires the broad screening of individuals. The tests that are currently being used are labor intensive and must be run in laboratory facilities by trained individuals. To identify and isolate infected individuals and limit the asymptomatic spread of SARS-CoV-2, diagnostic tests need to be inexpensive, easy to execute, and generate quick results. Lateral Flow Immunoassays (LFIAs) are being investigated as potential assays for mass testing. These assays, which work in a manner like home pregnancy tests, offer a means of quickly testing for the presence of SARS-CoV-2 antigens or anti-SARS-CoV-2 IgG or IgM antibodies. LFIAs are easy to use, quicker to run, and cost less than the PCR diagnostic tests currently being used, making them ideal for mass testing, specifically in countries that lack extensive laboratory facilities.

The performance of LFIAs is dependent on its capture antibodies. LFIA capture antibodies are in contact with the antigens for only a short period of time. Thus, the generation of high performing LFIAs needs high-affinity capture antibodies. R&D Systems offers antibodies for LFIA development that display the highest affinity toward SARS-CoV-2 antigens or antibodies.

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Additionally, visit our main Antibodies for Coronavirus Research page for more information on our antibodies and related resources for SARS-CoV-2 research.

Custom and Bulk Antibodies for COVID-19 Research

  • Thousands of non-catalog antibodies ready for immediate screening
  • Screen in your assays and assay development
  • All antibodies scalable to bulk quantities

Contact Our Custom Antibody Services

 

R&D Systems Anti-SARS-CoV-2 Antibodies for SARS-CoV-2 Immunoassay Development

The major method currently being used to determine active infections is PCR testing, which detects viral RNA. However, due to several major drawbacks of PCR assays, alternative tests, such as antigen testing, are being explored. Antigen tests are immunoassays that detect the presence of a viral proteins, which implies a current viral infection. R&D Systems offers antibodies directed against SARS-CoV-2 antigens, such as the S1 and S2 subunits, as well as the Nucleocapsid Protein, for development of SARS-CoV-2 antigen tests.

 

SARS-CoV-2 Antigen Antibody Catalog # Clone Other Information
SARS-CoV-2 Spike RBD MAB10540 1034522  
MAB105401 1034515  
MAB105801 1035419  
MAB105802 1035423  
LMAB10541* VHH72 Llamabody-Camelid Antibody
MAB105406 1035240  
MAB10580 1035414  
MAB105806 1035709  
MAB105807 1035740  
MAB105808 1035753  
Spike S1 Subunit

MAB105403

1035206 Conjugates Available

MAB105405

1035224  

MAB105805

1035226 Conjugates Available

MAB10637

1035341  

MAB105407*

2811A  
Spike S2 Subunit

MAB10557

1034617  

MAB106371

1037111  
Nucleocapsid Protein

MAB10474

1035111  

MAB104741

1035145  

MAB104742

1035143  

 

R&D Systems Anti-Human Secondary Antibodies for IgG and IgM Immunoassay Development

Serological analysis detects antibody responses initiated by the SARS-CoV-2 virus and is used to determine past SARS-CoV-2 infections. These assays can also have an important role in assessing efficacy of potential vaccines. Similar to other viruses, infection with SARS-CoV-2 induces antibody production. IgM antibodies can be detected early after SARS-CoV-2 infection, followed by the appearance of IgG antibodies. It is believed that testing for the humoral immune response to SARS-CoV-2 is key for surveying viral spread as it can provide valuable information such as the proportion of the population that may have previously been infected. R&D Systems offers secondary antibodies for the development of IgG and IgM immunoassays.

Molecule Antibody Catalog # Clone Host Other Information
IgG Fc MAB110 97924 Mouse Conjugates Available
MAB11012* 1268C Rabbit Conjugates Available
IgG MAB11013* 1268A Rabbit  
MAB1101* 1268B Rabbit  
F0135 Polyclonal Goat APC-Conjugated
G-101-C-ABS Polyclonal Goat H+L Antibody, X Absorbed

MAB105405

1035224    
IgM G-105-C Polyclonal Goat  
MAB9435* 2123A Rabbit  
MAB94351* 2123C Rabbit  

*Indicates a recombinant monoclonal antibody

 

Detection of Human IgG1. A recombinant human IgG1 protein was serially diluted 2-fold and captured by a Mouse Anti-Human IgG1 Monoclonal Antibody (Catalog # MAB9894) coated on a Clear Polystyrene Microplate (Catalog # DY990). A Rabbit Anti-Human IgG Monoclonal Antibody (Catalog # MAB11012) was biotinylated and incubated with the plate for binding to the captured protein. Detection of the standard curve was achieved by incubating the plate with Streptavidin-HRP (Catalog # DY998), followed by the Substrate Solution (Catalog # DY999) and then the Stop Solution (Catalog # DY994) to stop the enzymatic reaction.

R&D Systems Rabbit Anti-Human IgG Monoclonal Antibody detects recombinant human IgG1.

 

Detection of Human IgM. A recombinant human IgM protein was serially diluted 2-fold and captured by a Rabbit Anti-Human IgM Monoclonal Antibody (Catalog # MAB9435) coated on a Clear Polystyrene Microplate (Catalog # DY990). A Rabbit Anti-Human IgM Monoclonal Antibody (Catalog # MAB94351) was biotinylated and incubated with the plate for binding to the captured protein. Detection of the standard curve was achieved by incubating the plate with Streptavidin-HRP (Catalog # DY998), followed by the Substrate Solution (Catalog # DY999) and then the Stop Solution (Catalog # DY994) to stop the enzymatic reaction.

R&D Systems Rabbit Anti-Human IgM Recombinant Monoclonal Antibody detects recombinant human IgM.

 

Why Choose R&D Systems Brand Antibodies for SARS-CoV-2 Immunoassay Development

We are the Experts in Producing Antibodies for Assay Development

R&D Systems has over 30 years of experience in developing antibodies. These antibodies are used by our development scientists to produce our wide range of immunoassay products, including our Quantikine® ELISA kits, the industry’s gold standard ELISAs.

 

Our Antibodies are Scalable to Gram Quantities

Because we manufacture the antibodies we sell, we can produce large quantities of your antibody of interest. Bulk ordering of your antibody means you will have a long-term supply of a reliably performing product, thus minimizing the likelihood that you will experience inconsistent performance of your immunoassay.

 

We are Committed to Providing Quality Products

Our quality standards are unmatched in the industry. Unlike our competition, we can develop and manufacture most of our antibodies in-house. This allows us to conduct quality tests that are not available to most life science reagent companies and thus offer consistently performing, high-quality antibodies to the biomedical field. Additionally, all our products are manufactured under compliance with ISO 9001:2008 and/or ISO 13485:2003/CMDR guidelines. To learn more about our commitment to quality, visit our Quality page and/or Review Our Certifications.

 

Testimonials

View a few examples of how our antibodies have been used to developed assays for multiple platforms.

To analyze the affinity of IFN-α2b and a recombinant IFN-α like anti-tumor/viral protein, Novaferon, for IFNR2, a surface plasmon resonance assay was developed utilizing an immobilized goat anti-human IgG Fc antibody (G-101-C-ABS, R&D Systems) to capture Human IFNR2-Fc, which was then incubated with the two compounds.

Cancer Cell International
Volume14, Article ID 24467885

An ELISA using a polyclonal goat anti-human IgG capture antibody (G-101-C-ABS, R&D Systems) and a mouse monoclonal anti-human IgG Fc detection antibody (MAB110, R&D Systems) was developed to determine mAb penetration in xenograft tumors following treatment with JO-1, a small recombinant adenovirus serotype 3-derived protein.

Cancer Research
Volume 71, Article ID 21990319