APP Antibody

Catalog #: PPS044 Datasheet / COA / SDS

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PPS044

Detection of Amyloid Precursor Protein by Western Blot.

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APP Antibody Summary

Species Reactivity

Human, Mouse, Rat, Canine, Chicken, Primate

Specificity

This antibody is specific for the ~120 kDa APP in Western blots of rat brain.

Source

Polyclonal Rabbit IgG

Purification

Antigen Affinity-purified

Immunogen

APP

Formulation

100 μL in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 μg/mL BSA and 50% glycerol.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

1:1000 dilution

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Amyloid Precursor Protein by Western Blot. Western blot of rat hippocampal lysate showing specific immunolabeling of the approximately 120 kDa APP protein.

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Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

For long-term storage, ≤ ‑20° C is recommended. Product is stable at ≤ ‑20° C for at least 1 year.

Background: APP

APP, or amyloid precursor protein, is a heparin-binding, 120 kDa type I transmembrane glycoprotein that belongs to the APP family of proteins. It is highly conserved across species, showing at least 97% amino acid (aa) identity between human, monkey, rat, mouse and dog. There are almost a dozen potential alternate splice forms for human APP. The neuronal (or standard) form is referred to as APP695. The 695 includes a 17 aa signal peptide and 678 aa mature region. This form contains a multidomain, 610 aa extracellular region (ECD), a 24 aa transmembrane segment (aa 625 - 648), and a short 47 aa cytoplasmic tail. Longer forms of APP, APP751 and APP770 are characterized by the presence of a 57 aa KPI, or Kunitz protease inhibitor domain in the ECD. This has protease-inhibition activity and may be involved in memory. Membrane-bound APP695 undergoes two-stage proteolytic processing. There is an initial cleavage by either an alpha - or beta -secretase, followed by cleavage by a gamma -secretase. The choice of alpha - or beta -secretase may depend upon the APP glycosylation pattern or phosphorylation events. alpha -Secretase cleavage occurs between K612 and L613. This releases a soluble APPa piece that shows proliferative activity with EGF family members on neural progenitor cells and keratinocytes. beta -secretase cleaves between M596 and D597. When followed by gamma -secretase cleavage following position V637, or A639, small 40 or 42 aa A beta peptides are released that may contribute to the formation of amyloid plaques.

References

Suzuki, T. et al. (2006) J. Biochem. 139:949.
Chen, Y. and B.L. Tang (2006) Biochem. Biophys. Res. Commun. 341:1.
Reinhard, C. et al. (2005) EMBO J. 24:3996.
Mendendez-Gonzales, M. et al. (2005) Neurodegenerative Dis. 2:227.
Nakagawa, K. et al. (2006) J. Neurochem. 96:924.
Kang, J. et al. (1987) Nature 325:733.

Long Name

Amyloid Precursor Protein

Entrez Gene IDs

351 (Human); 11820 (Mouse)

Alternate Names

amyloid beta (A4) precursor protein-binding, family B, member 2; amyloid beta A4 precursor protein-binding family B member 2; Amyloid beta precursor protein; Amyloid beta; APP; beta Amyloid; Protease Nexin II