Avi Tag Antibody Summary
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Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of Avi Tag by Western Blot. Western blot shows recombinant human VEGF-165 Avi Tag protein and recombinant human OX40 His Tag protein (negative control). PVDF membrane was probed with 1 µg/mL of Rabbit Anti-Avi Tag Monoclonal Antibody (Catalog # MAB10546) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (HAF008). A specific band was detected for recombinant human VEGF-165 Avi Tag protein at approximately 26 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.
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Detection of Avi Tag by Simple WesternTM. Simple Western lane view shows recombinant OX40 Avi Tag protein, loaded at 0.2 mg/mL. A specific band was detected for recombinant OX40 Avi Tag at approximately 74 kDa (as indicated) using 20 µg/mL of Rabbit Anti-Avi Tag Monoclonal Antibody (Catalog # MAB10546). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Product Datasheets
FAQs
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Reconstitution Buffers
Secondary Antibodies
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