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Human AAK1 Antibody

Catalog #: MAB6886
1 Citation Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB6886
MAB6886-SP
Detection of Human and Mouse AAK1 by Western Blot.
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Citations (1)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human AAK1 Antibody Summary

Species Reactivity
Human
Specificity
Detects human AAK1 in direct ELISAs.
Source
Monoclonal Mouse IgG2B Clone # 702425
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant human AAK1
Ser704-Ile822
Accession # Q2M2I8
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human and Mouse AAK1 antibody by Western Blot. View Larger

Detection of Human and Mouse AAK1 by Western Blot. Western blot shows lysates of IMR-32 human neuroblastoma cell line, Neuro-2A mouse neuroblastoma cell line, and L1.2 mouse pro-B cell line. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human AAK1 Monoclonal Antibody (Catalog # MAB6886) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for AAK1 (Long Form) at approximately 140 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: AAK1

AP2-associated protein kinase 1 (AAK1) is a 100 kDa intracellular kinase that binds and phosphorylates NUMB and the m2 subunit of AP2. NUMB and AP2 are adaptor proteins which are involved in the assembly and sorting of clathrin coated pits during receptor-mediated endocytosis. AAK1 contains one catalytic domain (aa 46‑315) and a C-terminal clathrin-binding region. Alternate splicing of human AAK1 generates a 145 kDa long isoform that contains an additional clathrin-binding domain. Within aa 704‑822, human AAK1 shares 95% aa sequence identity with mouse and rat AAK1.

Long Name
AP2-associated Protein Kinase 1
Entrez Gene IDs
22848 (Human); 269774 (Mouse); 500244 (Rat)
Alternate Names
AAK1; Adaptor-associated kinase 1; AP2 associated kinase 1; AP2-associated protein kinase 1; DKFZp686F03202; DKFZp686K16132; EC 2.7.11.1; FLJ23712; FLJ25931; FLJ31060; FLJ45252; KIAA1048FLJ42882; MGC138170; MGC164568; MGC164570

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Citation for Human AAK1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Regulation of senescence escape by the cdk4-EZH2-AP2M1 pathway in response to chemotherapy
    Authors: M Le Duff, J Gouju, B Jonchère, J Guillon, B Toutain, A Boissard, C Henry, C Guette, E Lelièvre, O Coqueret
    Cell Death Dis, 2018-02-07;9(2):199.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot

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