Human ABCB5 Antibody

Catalog #: AF5799 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF5799
AF5799-SP

Detection of Human ABCB5 by Western Blot.

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human ABCB5 Antibody Summary

Species Reactivity

Human

Specificity

Detects human ABCB5 in direct ELISAs and Western blots.

Source

Polyclonal Sheep IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human ABCB5
Ile141-Val247
Accession # Q2M3G0

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human ABCB5 by Western Blot. Western blot shows lysates of Bowes human melanoma cell line and K562 human chronic myelogenous leukemia cell line. PVDF Membrane was probed with 1 µg/mL of Human ABCB5 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5799) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for ABCB5 at approximately 100-110 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: ABCB5

ABCB5 (ATP-binding cassette, subfamily B [DR/TAP] member 5) is a 90 kDa (predicted MW) member of the human P-glycoprotein family of molecules. It is expressed by CD133+ pluripotent pigment stem cells, where it serves as a marker for cells that show a high incidence of polyploidy. Human ABCB5 is 812 amino acids (aa) in length. It is possibly a five transmembrane protein with a 247 aa N-terminal extracellular domain (ECD) (aa 1-247) and a 283 aa C-terminal cytoplasmic region (aa 530-812). The ECD contains one ABC transporter (aa 2-177), while the cytoplasmic region contains a second ABC transporter (aa 570-808). There are two potential splice forms. One shows a seven aa substitution for aa 125-812, while another possesses an alternative start site 445 aa upstream of the standard site. Over aa 141-247, human ABCB5 shares 64% aa identity with mouse ABCB5.

Long Name

ATP-binding Cassette, Sub-family B (MDR/TAP), Member 5

Entrez Gene IDs

340273 (Human); 77706 (Mouse); 314537 (Rat)

Alternate Names

ABCB5 P-gp; ABCB5; ABCB5alpha; ABCB5beta; ATP-binding cassette protein; ATP-binding cassette sub-family B member 5; ATP-binding cassette, sub-family B (MDR/TAP), member 5; EC 3.6.3; EC 3.6.3.44; EST422562; P-glycoprotein ABCB5