Human ABCG2 Biotinylated Antibody

Catalog #: BAM995
1 Citation Datasheet / COA / SDS

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BAM995

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Human ABCG2 Biotinylated Antibody Summary

Species Reactivity

Human

Specificity

Detects human ABCG2.

Source

Monoclonal Mouse IgG_2B Clone # 5D3

Purification

Protein A or G purified from ascites

Immunogen

3T3 cells transduced with human ABCG2

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Label

Biotin

Applications

Recommended Concentration

Sample

Flow Cytometry

2.5 µg/10⁶ cells

Human MCF-7 cell line

Immunocytochemistry

5-25 µg/mL

Immersion fixed A549 human lung carcinoma cell line

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunocytochemistry

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ABCG2 in A549 Human Cell Line. ABCG2 was detected in immersion fixed A549 human lung carcinoma cell line using Mouse Anti-Human ABCG2 Biotinylated Monoclonal Antibody (Catalog # BAM995) at 5 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Streptavidin (red; NL999) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. Staining was performed using our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: ABCG2

Hematopoietic stem cells are known to express a membrane transporter molecule, known as P-glycoprotein (Pgp), that is encoded by the multidrug resistance gene 1 (MDR1) (1, 2). Expression of Pgp appears to confer a proliferative advantage to stem cells through its anti-apoptotic effects (3, 4). An additional transporter molecule known as ABCG2 (ATP-binding cassette gene 2) or Bcrp1 (Breast cancer resistance protein 1), first identified in a breast cancer cell line (5), is expressed on stem cells (6). ABCG2 belongs to a family of molecules that span the cell membrane six times and can exist as either homo or hetero dimers linked by a short intracellular flexible linker region that plays an important role in the efflux of a wide range of substrates (7, 8). Although these transporter molecules have initially been thought to play a role in drug resistance, they have been found to have utility in better characterizing primitive stem cells. For example, the “side-population” of hematopoietic stem cells, characterized by their inability to retain high levels of the intracellular staining dyes Hoechst 33342 and Rhodamine 123, has been found to express high levels of ABCG2. Of interest is the observation that ABCG2 function has been linked to the efflux of the Hoechst dye (6). Furthermore, there is now evidence that this monoclonal can be used as a cell surface marker to identify hematopoietic stem cells within the bone marrow fraction of lineage negative cells (6). The expression of ABCG2 appears greatest on CD34^- cells and is downregulated with the acquisition of CD34 on the cell surface (6).

References

Chaudhary, P.M. and I.B. Roninson (1991) Cell 66:85.
Sorrentino, B.P. et al. (1995) Blood 86:491.
Pallis, M. and N. Russell (2000) Blood 95:2897.
Johnstone, R.W. et al. (1999) Blood 93:1075.
Doyle, L.A. et al. (1998) Proc. Natl. Acad. Sci. USA 95:15665.
Zhou, S. et al. (2001) Nat. Medicine 7:1028.
Hrycyna, C.A. et al. (1998) Biochem. 37:13660.
Bunting, K.D. (2002) Stem Cells 20:11.

Long Name

ATP-binding Cassette Transporter G2

Entrez Gene IDs

9429 (Human)

Alternate Names

ABC transporter; ABC15; ABCG2; ABCP; ABCPMGC102821; ATP-binding cassette sub-family G member 2; ATP-binding cassette transporter G2; ATP-binding cassette, sub-family G (WHITE), member 2; BCRP; BCRP1; BCRPMRX; BMDP; Breast cancer resistance protein; CD338 antigen; CD338; CDw338; EST157481; mitoxantrone resistance protein; Mitoxantrone resistance-associated protein; MXR; MXRMXR1; placenta specific MDR protein; Placenta-specific ATP-binding cassette transporter

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Citation for Human ABCG2 Biotinylated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

A novel two-step procedure to expand cardiac Sca-1+ cells clonally.
Authors: Tang YL, Shen L, Qian K, Phillips MI
Biochem. Biophys. Res. Commun., 2007-06-11;359(4):877-83.
Species: Mouse
Sample Types: Whole Cells
Applications: ICC