Home / ABCG2 / Human ABCG2 PE-conjugated Antibody

Human ABCG2 PE-conjugated Antibody

Catalog #: FAB995P
11 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
FAB995P
Detection of ABCG2 in MCF‑7 Human Cell Line by Flow Cytometry.
3 Images
Product Details
Citations (11)
FAQs
Reviews
Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human ABCG2 PE-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Detects human ABCG2 in flow cytometry and immunocytochemistry.
Source
Monoclonal Mouse IgG2B Clone # 5D3
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
3T3 cells transduced with human ABCG2
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Phycoerythrin (Excitation= 488 nm, Emission= 565-605 nm)

Applications

Recommended Concentration
Sample
Flow Cytometry
10 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of ABCG2 antibody in MCF-7 Human Cell Line antibody by Flow Cytometry. View Larger

Detection of ABCG2 in MCF‑7 Human Cell Line by Flow Cytometry. MCF-7 human breast cancer cell line was stained with Mouse Anti-Human ABCG2 PE-conjugated Monoclonal Antibody (Catalog # FAB995P, filled histogram) or isotype control antibody (IC0041P, open histogram). View our protocol for Staining Membrane-associated Proteins.

Flow Cytometry Detection of Porcine ABCG2 by Flow Cytometry View Larger

Detection of Porcine ABCG2 by Flow Cytometry Freshly isolated aortic pVICs express distinct cell surface markers.Freshly isolated aortic pVICs were stained with antibodies for CD31, OB–CDH, ABCG2, NG2, SSEA-4, and the corresponding control antibodies. Staining was quantified by flow cytometry. In the figure, the y-axis is fluorescence intensity and the x-axis is forward scattering. Percentage in the rectangular gates represents the fraction of positively stained cells. After subtracting the background, about 7.70% of these aortic pVICs stained positive for CD31, 4.71% stained positive for OB–CDH, 5.60% stained positive for ABCG2, 5.56% stained positive for NG2, and 6.59% stained positive for SSEA-4. Image collected and cropped by CiteAb from the following publication (https://dx.plos.org/10.1371/journal.pone.0069667), licensed under a CC-BY license. Not internally tested by R&D Systems.

Flow Cytometry View Larger

Detection of ABCG2 in RPMI 8226 cells by Flow Cytometry RPMI 8226 cells were stained with Mouse Anti-Human ABCG2 PE‑conjugated Monoclonal Antibody (Catalog # FAB995P, filled histogram) or isotype control antibody (Catalog # IC0041P, open histogram). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: ABCG2

Hematopoietic stem cells are known to express a membrane transporter molecule, known as P-glycoprotein (Pgp), that is encoded by the multidrug resistance gene 1 (MDR1) (1, 2). Expression of Pgp appears to confer a proliferative advantage to stem cells through its anti-apoptotic effects (3, 4). An additional transporter molecule known as ABCG2 (ATP-binding cassette gene 2) or Bcrp1 (Breast cancer resistance protein 1), first identified in a breast cancer cell line (5), is expressed on stem cells (6). ABCG2 belongs to a family of molecules that span the cell membrane six times and can exist as either homo or hetero dimers linked by a short intracellular flexible linker region that plays an important role in the efflux of a wide range of substrates (7, 8). Although these transporter molecules have initially been thought to play a role in drug resistance, they have been found to have utility in better characterizing primitive stem cells. For example, the “side-population” of hematopoietic stem cells, characterized by their inability to retain high levels of the intracellular staining dyes Hoechst 33342 and Rhodamine 123, has been found to express high levels of ABCG2. Of interest is the observation that ABCG2 function has been linked to the efflux of the Hoechst dye (6). Furthermore, there is now evidence that this monoclonal can be used as a cell surface marker to identify hematopoietic stem cells within the bone marrow fraction of lineage negative cells (6). The expression of ABCG2 appears greatest on CD34- cells and is downregulated with the acquisition of CD34 on the cell surface (6).

References
  1. Chaudhary, P.M. and I.B. Roninson (1991) Cell 66:85.
  2. Sorrentino, B.P. et al. (1995) Blood 86:491.
  3. Pallis, M. and N. Russell (2000) Blood 95:2897.
  4. Johnstone, R.W. et al. (1999) Blood 93:1075.
  5. Doyle, L.A. et al. (1998) Proc. Natl. Acad. Sci. USA 95:15665.
  6. Zhou, S. et al. (2001) Nat. Medicine 7:1028.
  7. Hrycyna, C.A. et al. (1998) Biochem. 37:13660.
  8. Bunting, K.D. (2002) Stem Cells 20:11.
Long Name
ATP-binding Cassette Transporter G2
Entrez Gene IDs
9429 (Human)
Alternate Names
ABC transporter; ABC15; ABCG2; ABCP; ABCPMGC102821; ATP-binding cassette sub-family G member 2; ATP-binding cassette transporter G2; ATP-binding cassette, sub-family G (WHITE), member 2; BCRP; BCRP1; BCRPMRX; BMDP; Breast cancer resistance protein; CD338 antigen; CD338; CDw338; EST157481; mitoxantrone resistance protein; Mitoxantrone resistance-associated protein; MXR; MXRMXR1; placenta specific MDR protein; Placenta-specific ATP-binding cassette transporter

Product Datasheets

You must select a language.

x
Product Datasheet
COA
SDS

Citations for Human ABCG2 PE-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

11 Citations: Showing 1 - 10
Filter your results:

Filter by:

  1. The acquisition order of leukemic drug resistance mutations is directed by the selective fitness associated with each resistance mechanism
    Authors: Leow, BCS;Kok, CH;Yeung, DT;Hughes, TP;White, DL;Eadie, LN;
    Scientific reports
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  2. Solute Carrier Family 29A1 Mediates In Vitro Resistance to Azacitidine in Acute Myeloid Leukemia Cell Lines
    Authors: MM Kutyna, S Loone, VA Saunders, DL White, CH Kok, DK Hiwase
    International Journal of Molecular Sciences, 2023-02-10;24(4):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  3. Physiologic expansion of human heart-derived cells enhances therapeutic repair of injured myocardium
    Authors: S Mount, P Kanda, S Parent, S Khan, C Michie, L Davila, V Chan, RA Davies, H Haddad, D Courtman, DJ Stewart, DR Davis
    Stem Cell Res Ther, 2019-11-04;10(1):316.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  4. Localization of the placental BCRP/ABCG2 transporter to lipid rafts: Role for cholesterol in mediating efflux activity
    Authors: JT Szilagyi, AM Vetrano, JD Laskin, LM Aleksunes
    Placenta, 2017-04-12;55(0):29-36.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  5. Acetazolamide potentiates the anti-tumor potential of HDACi, MS-275, in neuroblastoma
    Authors: R Bayat Mokh, N Baluch, M Ka Hon Tsu, S Kumar, T S Homayoun, K Aitken, B Das, S Baruchel, H Yeger
    BMC Cancer, 2017-02-24;17(1):156.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  6. Chronic inflammation and angiogenic signaling axis impairs differentiation of dental-pulp stem cells.
    Authors: Boyle M, Chun C, Strojny C, Narayanan R, Bartholomew A, Sundivakkam P, Alapati S
    PLoS ONE, 2014-11-26;9(11):e113419.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  7. Characterization of Cell Subpopulations Expressing Progenitor Cell Markers in Porcine Cardiac Valves
    Authors: Huan Wang, Balaji Sridhar, Leslie A. Leinwand, Kristi S. Anseth
    PLoS ONE
  8. Epstein-Barr virus latent membrane protein 1 induces cancer stem/progenitor-like cells in nasopharyngeal epithelial cell lines.
    Authors: Kondo S, Wakisaka N, Muramatsu M
    J. Virol., 2011-08-17;85(21):11255-64.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  9. The presence of a side population and its marker ABCG2 in human deciduous dental pulp cells.
    Biochem. Biophys. Res. Commun., 2010-08-20;400(0):334-9.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  10. Side-population cells in luminal-type breast cancer have tumour-initiating cell properties, and are regulated by HER2 expression and signalling.
    Authors: Nakanishi T, Chumsri S, Khakpour N, Brodie AH, Leyland-Jones B, Hamburger AW, Ross DD, Burger AM
    Br. J. Cancer, 2010-02-09;102(5):815-26.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  11. Effect of NOS1 regulating ABCG2 expression on proliferation and apoptosis of cervical cancer cells
    Authors: M Ding, H Zhang, L Liu, R Liang
    Oncol Lett, 2018-11-30;17(2):1531-1536.

FAQs

No product specific FAQs exist for this product, however you may

View all Antibody FAQs

Reviews for Human ABCG2 PE-conjugated Antibody

There are currently no reviews for this product. Be the first to review Human ABCG2 PE-conjugated Antibody and earn rewards!

Have you used Human ABCG2 PE-conjugated Antibody?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥2500 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review