Human ADAM33 Antibody

Catalog #: MAB5565 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB5565
MAB5565-SP

Detection of ADAM33 in A549 Human Cell Line by Flow Cytometry.

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human ADAM33 Antibody Summary

Species Reactivity

Human

Specificity

Detects human ADAM33 in direct ELISAs. In direct ELISAs, less than 5% cross‑reactivity with recombinant mouse ADAM33 is observed.

Source

Monoclonal Mouse IgG₁ Clone # 508101

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Chinese hamster ovary cell line CHO-derived recombinant human ADAM33
Val30-Leu501 (predicted)
Accession # Q9BZ11

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Immunoprecipitation

25 µg/mL

Conditioned cell culture medium spiked with Recombinant Human ADAM33, see our available Western blot detection antibodies

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Intracellular Staining by Flow Cytometry

2.5 µg/10⁶ cells

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Intracellular Staining by Flow Cytometry

Detection of ADAM33 antibody in A549 Human Cell Line antibody by Flow Cytometry.

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Detection of ADAM33 in A549 Human Cell Line by Flow Cytometry. A549 human lung carcinoma cell line was stained with Mouse Anti-Human ADAM33 Monoclonal Antibody (Catalog # MAB5565, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG F(ab')₂Secondary Antibody (Catalog # F0102B). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: ADAM33

ADAM33 is a widely expressed member of the ADAM family of transmembrane proteins. It contains a metalloprotease-like domain, disintegrin cell adhesion domain, and cysteine rich domain. ADAM33 is involved in the maintenance of airway function, and ADAM33 polymorphisms have been associated with asthma susceptibility and bronchial hyperresponsiveness. Within the mature ectodomain, human and mouse ADAM33 share 79% amino acid sequence identity.

Long Name

A Disintegrin and Metalloproteinase Domain 33

Entrez Gene IDs

80332 (Human); 110751 (Mouse)

Alternate Names

a disintegrin and metalloprotease 33; a disintegrin and metalloproteinase domain 33; ADAM 33; ADAM metallopeptidase domain 33; ADAM33; C20orf153; chromosome 20 open reading frame 153; disintegrin and metalloproteinase domain-containing protein 33; disintegrin and reprolysin metalloproteinase family protein; dJ964F7.1; DKFZp434K0521; EC 3.4.24.-; FLJ35308; FLJ36751; MGC149823; MGC71889