Human Akt3 Antibody

Catalog #: MAB14631 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB14631
MAB14631-SP

All Akt3 Antibodies

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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Akt3 Antibody Summary

Species Reactivity

Human

Specificity

Detects human Akt3 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human (rh) Akt1 or rhAkt2 is observed.

Source

Monoclonal Mouse IgG_2A Clone # 199822

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived recombinant human Akt3
Arg23-Ala101
Accession # NP_005456

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Intracellular Staining by Flow Cytometry

2.5 µg/10⁶ cells

MCF‑7 human breast cancer cell line fixed with paraformaldehyde and permeabilized with saponin

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Akt3

Akt3 (v-akt murine thymoma viral oncogene homolog 3), also known as protein kinase B gamma (PKB gamma ) and RAC-gamma serine/threonine kinase (RAC-PK-gamma ), is a member of the Akt family that also includes Akt1 (PKB alpha ) and Akt2 (PKB beta ). These highly homologous kinases are central in such diverse cellular processes as glucose uptake, cell cycle progression, and apoptosis. All three Akts contain an amino-terminal pleckstrin homology domain, a central kinase domain, and a carboxyl-terminal regulatory domain.

Long Name

v-Akt Murine Thymoma Viral Oncogene Homolog 3

Entrez Gene IDs

10000 (Human); 23797 (Mouse)

Alternate Names

Akt3; Akt-3; EC 2.7.11; EC 2.7.11.1; PKB gamma; PKBGDKFZp434N0250; PRKBG; RAC-gamma; v-akt murine thymoma viral oncogene homolog 3 (protein kinase B, gamma)