Home / ALCAM/CD166 / Human ALCAM/CD166 Antibody

Human ALCAM/CD166 Antibody

Catalog #: MAB6561
11 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB6561-SP
MAB6561-100
MAB6561-500
Product Details
Citations (11)
FAQs
Supplemental Products
Reviews
Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human ALCAM/CD166 Antibody Summary

Species Reactivity
Human
Specificity
Detects human ALCAM/CD166 in Western blots. Shows approximately 50% cross‑reactivity with recombinant mouse OCAM and no cross‑reactivity with recombinant human (rh) BCAM, rhEpCAM, rhMCAM, or rhNCAM-L1.
Source
Monoclonal Mouse IgG1 Clone # 105902
Purification
Protein A or G purified from ascites
Immunogen
Mouse myeloma cell line NS0-derived recombinant human ALCAM/CD166
Trp28-Ala526
Accession # Q13740
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
Recombinant Human ALCAM/CD166 Fc Chimera (Catalog # 656-AL) under non-reducing conditions only
Flow Cytometry
2.5 µg/106 cells
Human peripheral blood monocytes
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 

Human ALCAM/CD166 Sandwich Immunoassay

Recommended Concentration
Reagent
ELISA Capture (Matched Antibody Pair)
2-8 µg/mL 

Use in combination with:

Detection Reagent: Human ALCAM/CD166 Biotinylated Antibody (Catalog # BAF656)

Standard: Recombinant Human ALCAM Fc Chimera Protein, CF (Catalog # 656-AL)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Loading...
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: ALCAM/CD166

ALCAM, activated leukocyte cell adhesion molecule, is a type I membrane glycoprotein and a member of the immunoglobulin supergene family. It is also known as CD166, MEMD, SC-1/DM-GRASP/BEN in the chicken, and KG-CAM in the rat. ALCAM is expressed on thymic epithelial cells, activated B and T cells, and monocytes. ALCAM can bind itself homotypically and is also capable of binding CD6, NgCAM, and other, as of yet, unidentified brain proteins. The ALCAM/CD6 interaction may be involved in T cell development and T cell regulation. Additionally, ALCAM/CD6 and ALCAM/NgCAM interactions may play roles in the nervous system. ALCAM has also been observed to be upregulated on highly metastasizing melanoma cell lines and may play a role in tumor migration. ALCAM is a 583 amino acid (aa) protein consisting of a 27 aa signal peptide, a 500 aa extracellular domain, a 24 aa transmembrane domain and a 32 aa cytoplasmic domain. The extracellular domain of ALCAM contains 5 Ig-like domains.

References
  1. Bowen, M.A. et al. (1995) J. Exp. Med. 181:2213.
  2. Aruffo, A. et al. (1997) Immunol. Today 18:498.
  3. Degen, W.G. et al. (1998) Am. J. Pathol. 152:805.
Long Name
Activated Leukocyte Cell Adhesion Molecule
Entrez Gene IDs
214 (Human); 11658 (Mouse); 101867493 (Cynomolgus Monkey)
Alternate Names
activated leucocyte cell adhesion molecule; activated leukocyte cell adhesion moleculeFLJ38514; ALCAM; CD166 antigen; CD166; MEMDMGC71733

Product Datasheets

You must select a language.

x
Product Datasheet
COA
SDS

Citations for Human ALCAM/CD166 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

11 Citations: Showing 1 - 10
Filter your results:

Filter by:

  1. Selectin Binding Sites Are Involved in Cell Adhesive Properties of Head and Neck Squamous Cell Carcinoma
    Authors: U Valentiner, J Knips, R Pries, T Clauditz, A Münscher, G Sauter, B Wollenberg, U Schumacher
    Cancers (Basel), 2019-10-28;11(11):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  2. Fluoxetine induces direct inhibitory effects on mesenchymal stem cell?derived osteoprogenitor cells independent of serotonin concentration
    Authors: SM Koura, M Salama, M El-Hussiny, MEA Khalil, A Lotfy, SA Hassan, SA Gad Elhak, MA Sobh
    Mol Med Rep, 2019-02-01;0(0):.
    Species: Rat
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  3. Alternative splicing of ALCAM enables tunable regulation of cell-cell adhesion through differential proteolysis
    Authors: KE Hebron, EY Li, SA Arnold Egl, AK von Lersne, C Taylor, J Houkes, DK Flaherty, A Eskaros, TP Stricker, A Zijlstra
    Sci Rep, 2018-02-16;8(1):3208.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  4. ADAM10 new selective inhibitors reduce NKG2D ligand release sensitizing Hodgkin lymphoma cells to NKG2D-mediated killing
    Authors: Maria Raffaella Zocchi, Caterina Camodeca, Elisa Nuti, Armando Rossello, Roberta Venè, Francesca Tosetti et al.
    OncoImmunology
  5. Overexpression of activated leukocute cell adhesion molecule in gastric cancer is associated with advanced stages and poor prognosis and miR-9 deregulation
    Authors: MIN YE, YAN-LEI DU, YU-QIANG NIE, ZHI-WEI ZHOU, JIE CAO, YING-FEI LI
    Molecular Medicine Reports
  6. JAM-A and ALCAM are therapeutic targets to inhibit diapedesis across the BBB of CD14+CD16+ monocytes in HIV-infected individuals.
    Authors: Williams D, Anastos K, Morgello S, Berman J
    J Leukoc Biol, 2014-11-24;97(2):401-12.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  7. Elevated ALCAM shedding in colorectal cancer correlates with poor patient outcome.
    Authors: Hansen A, Freeman T, Arnold S, Starchenko A, Jones-Paris C, Gilger M, Washington M, Fan K, Shyr Y, Beauchamp R, Zijlstra A
    Cancer Res, 2013-03-28;73(10):2955-64.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  8. Relevance of activated leukocyte cell adhesion molecule (ALCAM) in tumor tissue and sera of cervical cancer patients.
    Authors: Ihnen M, Kress K, Kersten JF, Kilic E, Choschzick M, Zander H, Muller V, Mahner S, Janicke F, Woelber L, Milde-Langosch K
    BMC Cancer, 2012-04-04;12(0):140.
    Species: Human
    Sample Types: Serum
    Applications: ELISA Development
  9. Label-Free Biomarker Sensing in Undiluted Serum with Suspended Microchannel Resonators
    Authors: Marcio G. von Muhlen, Norman D. Brault, Scott M. Knudsen, Shaoyi Jiang, Scott R. Manalis
    Analytical Chemistry
  10. Activated leukocyte cell adhesion molecule: a novel biomarker for breast cancer.
    Authors: Kulasingam V, Zheng Y, Soosaipillai A, Leon AE, Gion M, Diamandis EP
    Int. J. Cancer, 2009-07-01;125(1):9-14.
    Species: Human
    Sample Types: Serum
    Applications: ELISA Development
  11. Phenotypic characterization of human colorectal cancer stem cells.
    Authors: Dalerba P, Dylla S, Park I, Liu R, Wang X, Cho R, Hoey T, Gurney A, Huang E, Simeone D, Shelton A, Parmiani G, Castelli C, Clarke M
    Proc Natl Acad Sci U S A, 2007-06-04;104(24):10158-63.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry

FAQs

No product specific FAQs exist for this product, however you may

View all Antibody FAQs
Loading...

Reviews for Human ALCAM/CD166 Antibody

There are currently no reviews for this product. Be the first to review Human ALCAM/CD166 Antibody and earn rewards!

Have you used Human ALCAM/CD166 Antibody?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥2500 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review