Human alpha 1B-Glycoprotein Antibody

Catalog #: MAB7757 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB7757
MAB7757-SP

Detection of Human alpha 1B-Glycoprotein by Western Blot.

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human alpha 1B-Glycoprotein Antibody Summary

Species Reactivity

Human

Specificity

Detects human alpha 1B-Glycoprotein in ELISAs and Western blot.

Source

Monoclonal Mouse IgG_2B Clone # 875431

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

HEK293 human embryonic kidney cell line transfected with human alpha 1B-Glycoprotein
Ala22-Ser495
Accession # P04217

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

2 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human alpha 1B-Glycoprotein by Western Blot. Western blot shows human plasma. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human a 1B-Glycoprotein Monoclonal Antibody (Catalog # MAB7757) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for a 1B-Glycoprotein at approximately 75 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: alpha 1B-Glycoprotein

Alpha-1 B-glycoprotein (A1BG), also known as C44 (in rodent) is a 68-80 kDa monomeric plasma glycoprotein member of the Immunoglobulin superfamily of molecules. It is expressed by multiple cell types, principally hepatocytes in response to circulating growth hormone. Initially thought to act as an MMP inhibitor, A1BG is now known to bind to CRISP3, a distant member of a family of venomous molecules. CRISP3 is widely expressed and possesses no toxic properties. But it may play a key role in fertilization, where it temporarily blocks the reaction of PMNs to sperm in the uterus, thus increasing sperm lifespan and the likelihood of fertilization. Within this context, A1BG may regulate CRISP3 availability. A1BG has also been investigated as a biomarker in various cancers. Mature human A1BG is 474 amino acids (aa) in length (aa 22-495) and contains five V-type Ig-like domains. There is one isoform variant that utilizes Met123 as an alternative start site. Full-length human A1BG (aa 22-495) shares only 44% aa sequence identity with mouse A1BG.

Entrez Gene IDs

1 (Human); 117586 (Mouse)

Alternate Names

A1B; A1BG; ABG; alpha 1BGlycoprotein; alpha 1B-Glycoprotein; Alpha-1-B glycoprotein;alpha1B-glycoprotein;Alpha-1B-glycoprotein;alpha-1-B-glycoprotein;DKFZp686F0970;epididymis secretory sperm binding protein Li 163pA;HEL-S-163pA; GAB