Human APCDD1 Antibody

Catalog #: MAB10501 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB10501-100
MAB10501-SP

Detection of APCDD1 on SW480 Cell Line by Flow Cytometry.

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All APCDD1 Antibodies

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human APCDD1 Antibody Summary

Species Reactivity

Human

Specificity

Detects human APCDD1 in direct ELISAs.

Source

Recombinant Monoclonal Rabbit IgG Clone # 2584B

Purification

Protein A or G purified from cell culture supernatant

Immunogen

Mouse myeloma cell line, NS0-derived human APCDD1
Leu27-His492
Accession # Q8J025

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Flow Cytometry

0.25 µg/10⁶ cells

SW480 human colorectal adenocarcinoma cell line

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

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Detection of APCDD1 on SW480 Cell Line by Flow Cytometry. SW480 human colorectal adenocarcinoma cell line was stained with Rabbit anti-Human APCDD1 Monoclonal Antibody (Catalog # MAB10501, filled histogram) or Rabbit IgG control Antibody (MAB1050, open histogram) followed by anti-Rabbit IgG Allophycoerythrin-conjugated Secondary Antibody (F0111). Staining was performed using our Staining Membrane-Associated Proteins protocol.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: APCDD1

APCDD1 (Adenomatosis Polyposis Coli Down-Regulated 1) is a membrane bound glycoprotein that is an endogenous inhibitor of the Wnt signaling pathway (1, 2). Inhibition of Wnt signaling by APCDD1 plays a role in adipocyte differentiation as well as pathogenesis of disease (2-4). Mature human APCDD1 consists of a 465 amino acid (aa) extracellular domain (ECD), a 19 aa transmembrane domain, and a 1 aa cytoplasmic region. Human APCDD1 shares a 95% and 94% amino acid sequence similarity with rat and mouse respectively. APCDD1 interacts in vitro with Wnt-3a and LRP5 (5). It is expressed in a broad repertoire of cell types which might regulate a diversity of biological processes controlled by Wnt signaling, including breast cancer cell invasion (6), osteogenic differentiation of human dental follicle cells (7), vascular remodeling and barrier maturation of retinal blood vessels (4) and hair follicle miniaturization (5). Extracellular domain of APCDD1 has been shown to co-immunoprecipitate with recombinant Wnt-3a and LRP5 (1), suggesting that APCDD1 can modulate the Wnt pathway by potential interactions with Wnt-3a and LRP5 at the cell surface.

References

Mazzoni, J. et al. (2017) Neuron. 96:1055.
Yiew, N.K.H. et al. (2017) J. Biol. Chem. 292:6312.
Shimomura, Y. (2010) Nature 464:1043.
Kandimalla, R. (2017) Oncogenesis 6:e308.
Yutaka Shimomura, et al. (2010) Nature 464:1043.
Sung-Gook CHO, (2017) Oncology Letters 14:4845.
Viale-Bouroncle S. et al. (2015) Biochem Biophys Res Commun 457(3):314.

Long Name

APC Down Regulated 1

Entrez Gene IDs

147495 (Human); 494504 (Mouse); 689616 (Rat)

Alternate Names

Adenomatosis polyposis coli down-regulated 1 protein; adenomatosis polyposis coli down-regulated 1; APC Down Regulated 1; APCDD1; B7323; DRAPC1; HYPT1; protein APCDD1