Human APLP-1 Antibody

Name: Human APLP-1 Antibody
Brand: R&D Systems
SKU: MAB3129
Rating: 5 (1 reviews)

Catalog #: MAB3129 1 Review Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB3129
MAB3129-SP

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human APLP-1 Antibody Summary

Species Reactivity

Human

Specificity

Detects human APLP-1 in direct ELISAs and Western blots. In direct ELISAs and Western blots, 100% cross‑reactivity with recombinant mouse APLP‑1 is observed and no cross-reactivity with recombinant human APLP‑2 is observed.

Source

Monoclonal Mouse IgG_2B Clone # 354008

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Mouse myeloma cell line NS0-derived recombinant human APLP-1
Gln34-Glu580 (predicted)
Accession # P51693

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Immunocytochemistry

8-25 µg/mL

Immersion fixed SH‑SY5Y human neuroblastoma cell line and TT human medullary thyroid cancer cell line

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunocytochemistry

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APLP‑1 in SH‑SY5Y Human Cell Line. APLP‑1 was detected in immersion fixed SH‑SY5Y human neuroblastoma cell line (positive staining) and MOLT‑4 human acute lymphoblastic leukemia cell line (negative staining) using Mouse Anti-Human APLP‑1 Monoclonal Antibody (Catalog # MAB3129) at 25 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. Staining was performed using our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Immunocytochemistry

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APLP‑1 in TT Human Cell Line. APLP‑1 was detected in immersion fixed TT human medullary thyroid cancer cell line (positive staining) and MOLT‑4 human acute lymphoblastic leukemia cell line (negative staining) using Mouse Anti-Human APLP‑1 Monoclonal Antibody (Catalog # MAB3129) at 25 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. Staining was performed using our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: APLP-1

APLP-1 is a transmembrane metalloprotein that is expressed in central neurons. Similar to APP and APLP-2, APLP-1 is susceptible to cleavage by various secretases, generating multiple fragments from the extracellular and intracellular domains. These include peptides similar to the amyloidogenic A beta peptides and a cytoplasmic fragment that associates with Fe65 family proteins and functions as a transcriptional activator. The extracellular domain contains heparin and collagen binding regions and shares 89% amino acid sequence identity between human and mouse.

Long Name

Amyloid-like Protein 1

Entrez Gene IDs

333 (Human); 11803 (Mouse); 502317 (Rat)

Alternate Names

amyloid beta (A4) precursor-like protein 1; amyloid precursor-like protein 1; amyloid-like protein 1; APLP1; APLP-1; APLPAPLP-1