Human Apolipoprotein B/ApoB Antibody

Catalog #: MAB41242 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB41242-100
MAB41242-SP

Detection of Human Apolipoprotein B/ApoB by Western Blot.

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Apolipoprotein B/ApoB Antibody Summary

Species Reactivity

Human

Specificity

Detects human Apolipoprotein B/ApoB in direct ELISAs.

Source

Monoclonal Mouse IgG₁ Clone # 1008037

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived recombinant human Apolipoprotein B/ApoB
Ile3374-Val3600
Accession # P04114

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

See below

Simple Western

5 µg/mL

Human Plasma

Immunohistochemistry

5-25 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human Apolipoprotein B/ApoB by Western Blot. Western blot shows lysates of human plasma. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human Apolipoprotein B/ApoB Monoclonal Antibody (Catalog # MAB41242) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Apolipoprotein B/ApoB at approximately 500 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunohistochemistry

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Apolipoprotein B/ApoB in Human Liver. Apolipoprotein B/ApoB was detected in immersion fixed paraffin-embedded sections of human liver using Mouse Anti-Human Apolipoprotein B/ApoB Monoclonal Antibody (Catalog # MAB41242) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to plasma membrane in hepatocytes. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Simple Western

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Detection of Human Apolipoprotein B/ApoB by Simple Western^TM. Simple Western shows lysates of human plasma, loaded at 1:50. A specific band was detected for Apolipoprotein B/ApoB at approximately 315 kDa (as indicated) using 5 µg/mL of Mouse Anti-Human Apolipoprotein B/ApoB Monoclonal Antibody (Catalog # MAB41242). This experiment was conducted under reducing conditions and using the 66-440kDa separation system.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Apolipoprotein B/ApoB

Human ApoB (Apolipoprotein B-100) is a 550 kDa, secreted, palmitoylated glycoprotein that is part of LDL and VLDL particles. It is made by liver and is 4536 amino acids (aa) in length. It binds LDL to the ApoB/E receptor. Multiple forms of ApoB-100 exist; ApoB-25, 26, 29, 37, 39, 48, and 74. The numbers reflect each molecule’s predicted MW as a percentage of ApoB-100 (512 kDa). Amino acids 1206-1414 of the precursor were used for immunization. Based on likelihood of secretion, this antibody may recognize B26 (1297 aa), B37 (1728 aa), B39 (1799 aa), and B48 (2152 aa). Over the immunization polypeptide range, human ApoB-100 is 80% and 82% aa identical to mouse and dog ApoB-100, respectively.

Entrez Gene IDs

338 (Human); 238055 (Mouse); 54225 (Rat)

Alternate Names

Apo B-100; APOB; ApoB-100; ApoB-48; apolipoprotein B (including Ag(x) antigen); Apolipoprotein B; apolipoprotein B-100; apolipoprotein B48; FLDB; LDLCQ4; mutant Apo B 100