Human ASAHL/N-acylethanolamine-hydrolyzing Acid Amidase Antibody

Catalog #: MAB4494
1 Citation Datasheet / COA / SDS

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MAB4494
MAB4494-SP

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All ASAHL/N-acylethanolamine-hydrolyzing Acid Amidase Antibodies

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Citations (1)

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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human ASAHL/N-acylethanolamine-hydrolyzing Acid Amidase Antibody Summary

Species Reactivity

Human

Specificity

Detects human ASAHL/N‑acylethanolamine-hydrolyzing Acid A in direct ELISAs and Western blots. In direct ELISAs, 100% cross-reactivity with recombinant mouse ASAHL is observed and no cross-reactivity with recombinant human ASAH2 or recombinant mouse ASAH2 is observed.

Source

Monoclonal Mouse IgG₁ Clone # 511702

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Chinese hamster ovary cell line CHO-derived recombinant human ASAHL/N‑acylethanolamine-hydrolyzing Acid A isoform 1
Ser29-Lys359 (Val151Ile)
Accession # Q02083

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

Recombinant Human N-acylethanolamine-hydrolyzing Acid Amidase/ASAHL (Catalog # 4494‑AH)

Immunoprecipitation

25 µg/mL

Conditioned cell culture medium spiked with Recombinant Human N-acylethanolamine-hydrolyzing Acid Amidase/ASAHL (Catalog # 4494-AH), see our available Western blot detection antibodies

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Reconstitution Buffer Available

Reconstitution Buffer 1 (PBS)

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: ASAHL/N-acylethanolamine-hydrolyzing Acid Amidase

The human NAAA gene encodes N-acylethanolamine-hydrolyzing acid amidase, also known as ASAH-like protein (ASAHL), a fatty acid amidase with maximal activity at acidic pH (1). ASAHL hydrolyzes a number of N-acyl ethanolamines, including N-myristoyl-, N-stearoyl, N-oleoyl, and N-arachidonoyl, but is most active against N‑palmitoylethanolamine (2). ASAHL is a member of the cholylglycine hydrolase family of enzymes, and is structurally similar to acid ceramidase (3). ASAHL is both a lysosomal and a secreted enzyme, and like acid ceramidase, has been observed to be proteolytically processed during maturation (3). ASAHL can be distinguished from anandamide amidohydrolase by its lack of inhibition by methyl arachidonyl fluorophosphonate (2).

References

Hong, S.B. et al. (1999) Genomics 62:232.
Ueda, N. et al. (2001) J. Biol. Chem. 276:35552.
Tsuboi, K. et al. (2005) J. Biol. Chem. 280:11082.

Entrez Gene IDs

27163 (Human); 67111 (Mouse); 497009 (Rat)

Alternate Names

Acid ceramidase-like protein; ASAHL; ASAH-like protein; EC 3.5.1.-; NAAA; N-acylethanolamine acid amidase; Nacylethanolaminehydrolyzing Acid Amidase; N-acylethanolamine-hydrolyzing acid amidase; N-acylsphingosine amidohydrolase (acid ceramidase)-like; N-acylsphingosine amidohydrolase-like; PLT

Product Datasheets

Product Datasheet

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Related Research Areas

Citation for Human ASAHL/N-acylethanolamine-hydrolyzing Acid Amidase Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

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