Human BMP-10 Propeptide Antibody

Catalog #: AF3956
2 Citations Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF3956
AF3956-SP

All BMP-10 Antibodies

Product Details

Citations (2)

Supplemental Products

Reviews

Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human BMP-10 Propeptide Antibody Summary

Species Reactivity

Human

Specificity

Detects human BMP‑10 propeptide in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with mature recombinant human BMP-10 is observed.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

Mouse myeloma cell line NS0-derived recombinant human BMP‑10 propeptide (R&D Systems, Catalog # 3956-BP)
Ser20-Arg313
Accession # O95393

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

Recombinant Human BMP‑10 Propeptide (Catalog # 3956-BP)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: BMP-10

BMP-10, along with BMP-9, GDF-5, -6, and -7, belongs to a subgroup of sequence related TGF-beta superfamily proteins that signal through heterodimeric complexes composed of type I and type II BMP receptors (1 - 3). Proteolytic removal of the propeptide from the 60 kDa proprotein yields a 12 kDa mature BMP-10 which forms disulfide-linked non-glycosylated homodimers (4, 5). In transfectants, BMP-10 is secreted as a cleaved mature dimer, an uncleaved proform dimer, and an uncleaved proform monomer (4). The propeptide of human BMP-10 shares 82% amino acid sequence identity with mouse and rat proBMP-10 and 19% - 34% with the propeptides of human BMP-9, GDF-5, -6, and -7. BMP-10 is critical for the proper development of the heart and first appears at the onset of trabeculation and chamber formation (6 - 8). Homozygous BMP-10 knockout mice die in utero due to arrested cardiac development (7). BMP-10 is required for maintaining expression of the cardiogenic transcription factors NKX2.5 and MEF2C in developing myocardium and promoting the growth of embryonic cardiomyocytes (7, 9, 10). The BMP-10 mediated proliferation of these cells requires Notch signaling (11). NKX2.5 itself negatively regulates BMP-10 expression in cardiac myocytes (10). Multiple human congenital heart defects result from mutations in NKX2.5 and require BMP-10 expression (10). In mice, genetic knockout of ErbB leads to a similar phenotype but appears not to involve BMP-10, and knockout of the calcium channel subunit FKBP12 induces BMP-10 overexpression (7). BMP-10 in the postnatal heart promotes increased cardiomyocyte and heart size (8). BMP-10 has been shown to induce signaling through ALK-1, BMPR-IA, BMPR-IB, and BMPR-II in transfectants and non-cardiac cell lines (4, 5). A functional BMP-10 receptor in the heart has not yet been identified, although deletion of BMPR-IA or BMP-10 causes similar cardiac morphogenetic abnormalities (12).

References

Chen, D. et al. (2004) Growth Factors 22:233.
Miyazono, K. et al. (2005) Cytokine Growth Factor Rev. 16:251.
Schneider, M.D. et al. (2003) Cytokine Growth Factor Rev. 14:1.
Mazerbourg, S. et al. (2005) J. Biol. Chem. 280:32122.
David, L. et al. (2007) Blood 109:1953.
Neuhaus, H. et al. (1999) Mech. Dev. 80:181.
Chen, H. et al. (2004) Development 131:2219.
Chen, H. et al. (2006) J. Biol. Chem. 281:27481.
Srivastava, D. and E.N. Olson (2000) Nature 407:221.
Pashmforoush, M. et al. (2004) Cell 117:373.
Grego-Bessa, J. et al. (2007) Dev. Cell 12:415.
Gaussin, V. et al. (2002) Proc. Natl. Acad. Sci. 99:2878.

Long Name

Bone Morphogenetic Protein 10

Entrez Gene IDs

27302 (Human); 12154 (Mouse)

Alternate Names

BMP10; BMP-10; bone morphogenetic protein 10; MGC126783

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citations for Human BMP-10 Propeptide Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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