Key Product Details

Species Reactivity

Human

Applications

Western Blot, Simple Western, Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all Carbonic Anhydrase VI Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human Carbonic Anhydrase VI
Gln18-Asn308
Accession # EAW71606

Specificity

Detects human Carbonic Anhydrase VI in direct ELISAs and Western blots. In direct ELISAs, approximately 5% cross-reactivity with recombinant human (rh) Carbonic Anhydrase (CA) 5A and rhCA5B is observed and less than 1% cross-reactivity with rhCA1, rhCA2, rhCA3, rhCA4, rhCA7, rhCA8, rhCA9, rhCA10, rhCA12, rhCA13, and rhCA14 is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human Carbonic Anhydrase VI Antibody

Detection of Human Carbonic Anhydrase VI antibody by Western Blot.

Detection of Human Carbonic Anhydrase VI by Western Blot.

Western blot shows human saliva, human milk, and lysates of HSC-3 human oral squamous cell carcinoma cell line and LoVo human colorectal adenocarcinoma cell line. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Human Carbonic Anhydrase VI Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2939) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Carbonic Anhydrase VI at approximately 42 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Detection of Human Carbonic Anhydrase VI antibody by Simple WesternTM.

Detection of Human Carbonic Anhydrase VI by Simple WesternTM.

Simple Western lane view shows lysates of human saliva, loaded at 0.2 mg/mL. A specific band was detected for Carbonic Anhydrase VI at approximately 56 kDa (as indicated) using 1 µg/mL of Goat Anti-Human Carbonic Anhydrase VI Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2939) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Applications for Human Carbonic Anhydrase VI Antibody

Application
Recommended Usage

Immunoprecipitation

25 µg/mL
Sample: Conditioned cell culture medium spiked with Recombinant Human Carbonic Anhydrase VI (Catalog # 2939-CA), see our available Western blot detection antibodies

Simple Western

1 µg/mL
Sample: Human saliva

Western Blot

0.5 µg/mL
Sample: Human saliva, human milk, HSC-3 human oral squamous cell carcinoma cell line, and LoVo human colorectal adenocarcinoma cell line

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Carbonic Anhydrase VI

Carbonic Anhydrase catalyzes the reversible reaction of CO2 + H2O = HCO3- + H+, which is fundamental to many processes such as respiration, renal tubular acidification and bone resorption (1). Topics in a CA meeting (6th International Conference on the CAs, June 20‑25, 2003, Slovakia) ranged from the use of CAs as markers for tumor and hypoxia in the clinic, as a nutritional supplement in milk, and as a tool for CO2 removal and mosquito control in industry. Carbonic Anhydrase VI, also known as gustin and salivary Carbonic Anhydrase, is a zinc-metalloprotein that constitutes about 3% of human parotid saliva protein (2, 3). It was decreased in patients with loss of taste and pathological changes in taste buds (4). It is also an elementary component of milk. It plays an important role in normal growth and development of the infant alimentary tract (5).

References

  1. Hewett-Emmett, D. and R.E. Tashian (1996) Mol. Phylogenet. Evol. 5:50.
  2. Murakami, H. and Sly, W. S. (1987) J. Biol. Chem. 262:1382.
  3. Thatcher, B. J. et al. (1998) Biochem. Biophys. Res. Commun. 250:635.
  4. Hankin, R. I. et al. (1999) Am. J. Med. Sci. 318:380.
  5. Karhumaa, P. et al. (2001) Proc. Natl. Acad. Sci. USA. 98:11604.

Alternate Names

CA6, Gustin

Entrez Gene IDs

765 (Human); 298657 (Rat)

Gene Symbol

CA6

UniProt

EAW71606

Additional Carbonic Anhydrase VI Products

Product Documents for Human Carbonic Anhydrase VI Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human Carbonic Anhydrase VI Antibody

For research use only

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