Human CCR1 Antibody

Catalog #: MAB1451 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB1451-100
MAB1451-SP

Detection of CCR1 in NS0 Mouse Cell Line Transfected with Human CCR1 and eGFP by Flow Cytometry.

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human CCR1 Antibody Summary

Species Reactivity

Human

Specificity

Detects human CCR1 in direct ELISAs.

Source

Monoclonal Mouse IgG_2A Clone # 984303

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Mouse myeloma cell line NS0-derived recombinant human CCR1
Met1-Phe355
Accession # P32246

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Flow Cytometry

0.25 µg/10⁶ cells

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

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Detection of CCR1 in NS0 Mouse Cell Line Transfected with Human CCR1 and eGFP by Flow Cytometry. NS0 mouse myeloma cell line transfected with Human CCR1and eGFP was stained with Mouse Anti-Human CCR1 Monoclonal Antibody (Catalog # MAB1451, filled histogram) or Mouse IgG2A Isotype Control Antibody (Catalog # MAB033, open histogram) followed by Goat anti-Mouse IgG APC-conjugated Secondary Antibody (Catalog # F0101B). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CCR1

CCR1 is a 7 transmembrane domain chemokine receptor expressed on T cells, B cells, monocytes, dendritic cells, and eosinophils. It binds the chemokines CCL3, 4, 5, and 7.

Entrez Gene IDs

1230 (Human); 12768 (Mouse); 57301 (Rat)

Alternate Names

C-C chemokine receptor type 1; CC-CKR-1; CCR1; CCR-1; CD191 antigen; CD191; chemokine (C-C motif) receptor 1; CKR1; CKR-1; CMKBR1SCYAR1; CMKR1; HM145C-C CKR-1; LD78 receptor; Macrophage inflammatory protein 1-alpha receptor; MIP-1alpha-R; MIP1aR; RANTES receptor; RANTES-R