Human CD117/c-kit PE-conjugated Antibody

Catalog #: FAB332P
4 Citations Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
FAB332P-100
FAB332P-025

Detection of CD117/c-kit in TF‑1 Human Cell Line by Flow Cytometry.

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All CD117/c-kit Antibodies

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Citations (4)

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human CD117/c-kit PE-conjugated Antibody Summary

Species Reactivity

Human

Specificity

Detects human CD117/c-kit in ELISAs and Western blots. In sandwich immunoassays and Western blots, no cross-reactivity with recombinant mouse CD117 is observed.

Source

Monoclonal Mouse IgG₁ Clone # 47233

Purification

Protein A or G purified from ascites

Immunogen

S. frugiperda insect ovarian cell line Sf 21-derived recombinant human CD117/c-kit
Gln26-Thr520
Accession # P10721

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Label

Phycoerythrin (Excitation= 488 nm, Emission= 565-605 nm)

Applications

Recommended Concentration

Sample

Flow Cytometry

10 µL/10⁶ cells

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

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Detection of CD117/c-kit in TF‑1 Human Cell Line by Flow Cytometry. TF-1 human erythroleukemic cell line was stained with Mouse Anti-Human CD117/c-kit PE-conjugated Monoclonal Antibody (Catalog # FAB332P, filled histogram) or isotype control antibody (Catalog # IC002P, open histogram). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.

12 months from date of receipt, 2 to 8 °C as supplied.

Background: CD117/c-kit

Stem Cell Factor Receptor (CD117, the gene product of the c-kit proto-oncogene) and its ligand, Stem Cell Factor (SCF), also known as c-kit Ligand, Mast Cell Growth Factor, play essential roles in gametogenesis, melanogenesis, and hematopoiesis. The human SCF Receptor cDNA encodes a 972 amino acid (aa) residue precursor membrane protein with a 25 aa residue signal peptide (experimentally determined), a 495 aa residue extracellular domain, a 23 aa residue transmembrane segment, and a 429 aa residue cytoplasmic domain. SCF Receptor is a member of the type III subfamily of Receptor Tyrosine Kinases (RTK) that also includes the receptors for M-CSF, Flt-3, PDGF, and VEGF. All class III RTKs are characterized by the presence of five immunoglobulin-like domains in their extracellular region and a split kinase domain in their intracellular region. SCF binding induces receptor homodimerization and signal transduction. SCF Receptor is expressed in hematopoietic progenitor cells, normal B and T lymphocyte progenitor cells, mast cells, germ cells, melanocytes, neurons, glial cells, placenta, kidney, lung, and gut. In addition, SCF Receptor expression has also been reported in a number of human tumor cell lines. SCF Receptor can be proteolytically cleaved from the cell surface and high levels of soluble SCF Receptor has been detected in cell conditioned medium and human plasma. Recombinant soluble SCF Receptor binds SCF with high affinity and is a potent SCF antagonist.

Long Name

Stem Cell Factor Receptor

Entrez Gene IDs

3815 (Human); 16590 (Mouse); 64030 (Rat)

Alternate Names

CD117 antigen; CD117; ckit; c-kit; EC 2.7.10; EC 2.7.10.1; KIT; PBT; piebald trait; Proto-oncogene c-Kit; proto-oncogene tyrosine-protein kinase Kit; SCF R; SCFR; SCFRmast/stem cell growth factor receptor; soluble KIT variant 1; Tyrosine-protein kinase Kit; v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog; v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene-like protein

Product Datasheets

Product Datasheet

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Related Research Areas

Citations for Human CD117/c-kit PE-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
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c-Kit Induces Migration of Triple-Negative Breast Cancer Cells and Is a Promising Target for Tyrosine Kinase Inhibitor Treatment
Authors: JA López-Mejí, LF Tallabs-Ut, P Salazar-So, JC Mantilla-O, MA Sánchez-Ca, L Rocha-Zava
International Journal of Molecular Sciences, 2022-08-05;23(15):.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry
Role of endothelial colony forming cells (ECFCs) Tetrahydrobiopterin (BH4) in determining ECFCs functionality in coronary artery disease (CAD) patients
Authors: A Sen, A Singh, A Roy, S Mohanty, N Naik, M Kalaivani, L Ramakrishn
Scientific Reports, 2022-02-23;12(1):3076.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry
Cultivation and characterization of pterygium as an ex vivo study model for disease and therapy
Authors: N Josifovska, DJ Szabó, R Nagymihály, Z Veréb, A Facskó, K Eriksen, MC Moe, G Petrovski
Cont Lens Anterior Eye, 2017-05-24;0(0):.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry
Cultivation and characterization of cornea limbal epithelial stem cells on lens capsule in animal material-free medium.
Authors: Albert R, Vereb Z, Csomos K, Moe M, Johnsen E, Olstad O, Nicolaissen B, Rajnavolgyi E, Fesus L, Berta A, Petrovski G
PLoS ONE, 2012-10-09;7(10):e47187.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry