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Human CD14 Antibody

Catalog #: MAB3832
24 Citations 2 Reviews Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB3832-SP
MAB3832-100
MAB3832-500
Detection of Human CD14 by Western Blot.
3 Images
Product Details
Citations (24)
FAQs
Supplemental Products
Reviews (2)
Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human CD14 Antibody Summary

Species Reactivity
Human
Specificity
Detects human CD14 in direct ELISAs and Western blots.
Source
Monoclonal Mouse IgG1 Clone # 134620
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Chinese hamster ovary cell line CHO-derived recombinant human CD14
Thr20-Cys352
Accession # P08571
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
2 µg/mL
Human peripheral blood mononuclear cells (PBMC)
Flow Cytometry
0.25 µg/106 cells
See below
Immunohistochemistry
5-25 µg/mL
Immersion fixed paraffin-embedded sections of human tonsil
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 
Neutralization
Measured by its ability to neutralize lipopolysacharide (LPS)-induced TNF‑ alpha secretion in the THP-1 human acute monocytic leukemia cell line. At 10 µg/mL, this anti-hCD14 antibody will neutralize >60% of 0.5 ng/mL LPS-induced TNF-alpha secretion.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot View Larger

Detection of Human CD14 by Western Blot. Western blot shows lysates of human peripheral blood mononuclear cells (PBMC). PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human CD14 Monoclonal Antibody (Catalog # MAB3832) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (HAF018). A specific band was detected for CD14 at approximately 55 kDa (as indicated). This experiment was conducted under non-reducing conditions and using Western Blot Buffer Group 1.

Flow Cytometry Detection of CD14 antibody in Human PBMCs antibody by Flow Cytometry. View Larger

Detection of CD14 in Human PBMCs by Flow Cytometry. Human peripheral blood mononuclear cells (PBMCs) were stained with Mouse Anti-Human CD14 Monoclonal Antibody (Catalog # MAB3832) followed by Goat anti-Mouse IgG APC-conjugated Secondary Antibody (Catalog # F0101B). View our protocol for Staining Membrane-associated Proteins.

Immunohistochemistry View Larger

Detection of CD14 in human tonsil. CD14 was detected in immersion fixed paraffin-embedded sections of human tonsil using Mouse Anti-Human CD14 Monoclonal Antibody (Catalog # MAB3832) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cell surface in lymphocytes. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CD14

CD14 is a 55 kDa cell surface glycoprotein that is preferentially expressed on monocytes/macrophages. The human CD14 cDNA encodes a 375 amino acid (aa) residue precursor protein with a 19 aa signal peptide and a C-terminal hydrophobic region characteristic for glycosylphosphatidyinositol (GPI)-anchored proteins. Human CD14 has four potential N-linked glycosylation sites and also bears O-linked carbohydrates. The amino acid sequence of human CD14 is approximately 65% identical with the mouse, rat, rabbit, and bovine proteins. CD14 is a pattern recognition receptor that binds lipopolysaccharides (LPS) and a variety of ligands derived from different microbial sources. The binding of CD14 with LPS is catalyzed by LPS-binding protein (LBP). The toll-like-receptors have also been implicated in the transduction of CD14-LPS signals. Similar to other GPI-anchored proteins, soluble CD14 can be released from the cell surface by phosphatidyinositol-specific phospholipase C. Soluble CD14 has been detected in serum and body fluids. High concentrations of soluble CD14 have been shown to inhibit LPS-mediated responses. However, soluble CD14 can also potentiate LPS response in cells that do not express cell surface CD14.

References
  1. Wright, S.D. et al. (1990) Science 249:1431.
  2. Pugin, J. et al. (1993) Proc. Natl. Acad. Sci. USA 90:2744.
  3. Beutler, B. (2000) Current Opinion in Immunology 12:20.
  4. Stelter, F. (2000) Chem. Immunol. 74:25.
Entrez Gene IDs
929 (Human); 12475 (Mouse); 60350 (Rat); 100037938 (Porcine)
Alternate Names
CD14 antigen; CD14 molecule; CD14; monocyte differentiation antigen CD14; Myeloid cell-specific leucine-rich glycoprotein

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Citations for Human CD14 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

24 Citations: Showing 1 - 10
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  1. Identification and elucidation of cross talk between SLAM Family Member 7 (SLAMF7) and Toll-like receptor (TLR) pathways in monocytes and macrophages
    Authors: Choe U, Pham Q, Kim YS et al.
    Scientific reports
  2. Urate crystals induce macrophage PAF?AH secretion which is differentially regulated by TGF?1 and hydrocortisone
    Authors: D Yagnik, F Hills
    Mol Med Rep, 2018-07-26;0(0):.
  3. CCN1 is an opsonin for bacterial clearance and a direct activator of Toll-like receptor signaling
    Authors: JI Jun, LF Lau
    Nat Commun, 2020-03-06;11(1):1242.
  4. Dioleoylphosphatidylglycerol Inhibits Heat Shock Protein B4 (HSPB4)-Induced Inflammatory Pathways In Vitro
    Authors: Fowler TE, Choudhary V, Melnyk S et al.
    International journal of molecular sciences
  5. Transcriptional and translational‐uncoupling in regulation of the CXCL12 and its receptors CXCR4, 7 in THP‐1 monocytes and macrophages
    Authors: Lu Yu, Liangli Yu, Quynhchi Pham, Thomas T. Y. Wang
    Immunity, Inflammation and Disease
  6. Human iPSC-derived myelinating organoids and globoid cells to study Krabbe Disease
    Authors: Evans, LMP;Gawron, J;Sim, FJ;Laura Feltri, M;Marziali, LN;
    bioRxiv : the preprint server for biology
    Species: Human
    Sample Types: Organoid
    Applications: Immunohistochemistry, Immunocytochemistry
  7. DEC205 mediates local and systemic immune responses to Helicobacter pylori infection in humans
    Authors: M Kita, K Yokota, C Kageyama, S Take, K Goto, Y Kawahara, O Matsushita, H Okada
    Oncotarget, 2018-02-26;9(22):15828-15835.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  8. Engineering the geometrical shape of mesenchymal stromal cells through defined cyclic stretch regimens
    Authors: B Walters, T Uynuk-Ool, M Rothdiener, J Palm, ML Hart, JP Stegemann, B Rolauffs
    Sci Rep, 2017-07-26;7(1):6640.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  9. Detection of tissue factor-positive extracellular vesicles by laser scanning confocal microscopy
    Authors: Yohei Hisada
    Thromb. Res, 2016-12-23;150(0):65-72.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  10. Granzymes A and K differentially potentiate LPS-induced cytokine response
    Authors: Annette C Wensink
    Cell Death Discov, 2016-12-12;2(0):16084.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  11. Synergic effects of VEGF-A and SDF-1 on the angiogenic properties of endothelial progenitor cells
    Authors: Gabriela Odent Grig
    J Tissue Eng Regen Med, 2016-12-12;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  12. Extracellular Vesicle Proteins Associated with Systemic Vascular Events Correlate with Heart Failure: An Observational Study in a Dyspnoea Cohort.
    Authors: Zhang Y, Vernooij F, Ibrahim I, Ooi S, Gijsberts C, Schoneveld A, Sen K, den Ruijter H, Timmers L, Richards A, Jong C, Mazlan I, Wang J, Lam C, de Kleijn D
    PLoS ONE, 2016-01-28;11(1):e0148073.
    Species: Human
    Sample Types: Plasma
    Applications: ELISA Development
  13. Secreted Thrombospondin-1 Regulates Macrophage Interleukin-1beta Production and Activation through CD47.
    Authors: Stein E, Miller T, Ivins-O'Keefe K, Kaur S, Roberts D
    Sci Rep, 2016-01-27;6(0):19684.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  14. Sialylation of Campylobacter jejuni endotoxin promotes dendritic cell-mediated B cell responses through CD14-dependent production of IFN-beta and TNF-alpha.
    Authors: Huizinga R, van Rijs W, Bajramovic J, Kuijf M, Laman J, Samsom J, Jacobs B
    J Immunol, 2013-10-28;191(11):5636-45.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  15. Mycobacterium tuberculosis infection and tissue factor expression in macrophages.
    Authors: Kothari H, Rao L, Vankayalapati R, Pendurthi U
    PLoS ONE, 2012-09-24;7(9):e45700.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  16. Bovine lactoferrin counteracts Toll-like receptor mediated activation signals in antigen presenting cells.
    Authors: Puddu P, Latorre D, Carollo M, Catizone A, Ricci G, Valenti P, Gessani S
    PLoS ONE, 2011-07-25;6(7):e22504.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  17. Human mesenchymal stromal cells express CD14 cross-reactive epitopes.
    Authors: Pilz GA, Braun J, Ulrich C, Felka T, Warstat K, Ruh M, Schewe B, Abele H, Larbi A, Aicher WK
    Cytometry A, 2011-07-06;79(8):635-45.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  18. Iris pigment epithelial cells express a functional lipopolysaccharide receptor complex.
    Authors: Chui JJ, Li MW, Di Girolamo N, Chang JH, McCluskey PJ, Wakefield D
    Invest. Ophthalmol. Vis. Sci., 2009-12-17;51(5):2558-67.
    Species: Human
    Sample Types: Cell Lysates, Whole Cells
    Applications: Neutralization, Western Blot
  19. Functional dissection of an IFN-alpha/beta receptor 1 promoter variant that confers higher risk to chronic hepatitis B virus infection.
    Authors: Zhou J, Huang JD, Poon VK, Chen DQ, Chan CC, Ng F, Guan XY, Watt RM, Lu L, Yuen KY, Zheng BJ
    J. Hepatol., 2009-05-03;51(2):322-32.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  20. IL-1alpha and IL-1beta are endogenous mediators linking cell injury to the adaptive alloimmune response.
    Authors: Rao DA, Tracey KJ, Pober JS
    J. Immunol., 2007-11-15;179(10):6536-46.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  21. Borrelia burgdorferi-induced monocyte chemoattractant protein-1 production in vivo and in vitro.
    Authors: Zhao Z, McCloud B, Fleming R, Klempner MS
    Biochem. Biophys. Res. Commun., 2007-05-02;358(2):528-33.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  22. LPS/CD14 activation triggers SGLT-1-mediated glucose uptake and cell rescue in intestinal epithelial cells via early apoptotic signals upstream of caspase-3.
    Authors: Yu LC, Turner JR, Buret AG
    Exp. Cell Res., 2006-06-22;312(17):3276-86.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC, Neutralization
  23. Calcium Dobesilate Modulates PKCδ-NADPH Oxidase- MAPK-NF-kappa B Signaling Pathway to Reduce CD14, TLR4, and MMP9 Expression during Monocyte-to-Macrophage Differentiation: Potential Therapeutic Implications for Atherosclerosis
    Authors: Florence Njau, Hermann Haller
    Antioxidants (Basel)
  24. Toll-like Receptor-Mediated Responses by Placental Hofbauer Cells (HBCs): A Potential Pro-Inflammatory Role for Fetal M2 Macrophages*
    Authors: Omar M. Young, Zhonghua Tang, Tracy Niven-Fairchild, Serkalem Tadesse, Graciela Krikun, Errol R. Norwitz et al.
    American Journal of Reproductive Immunology

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Reviews for Human CD14 Antibody

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Human CD14 Antibody
By Anonymous on 10/12/2021
Application: WB Sample Tested: THP-1 human acute monocytic leukemia cell line Species: Human

Cells lysed in NP-40 lysis buffer. TBST-150mM NaCl. Blocked with 5% milk TBST. Antibody diluted in 2% milk TBST 1:100 (5ug/ml). Product works better under non-reducing conditions.

Western Blot Human CD14 Antibody MAB3832
Human CD14 Antibody
By Anonymous on 03/29/2021
Application: ELISA Sample Tested: wheat extracts Species: wheat

we use this antibody to see interactions with CD14 recombinant protein, it showed a very good affinity towards CD14 protein in direct ELISA.

ELISA Human CD14 Antibody MAB3832