Human CD157 Antibody

Catalog #: MAB47361 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB47361
MAB47361-SP

Detection of CD157 in Human Blood Granulocytes by Flow Cytometry.

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All CD157 Antibodies

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human CD157 Antibody Summary

Species Reactivity

Human

Specificity

Detects human CD157 in direct ELISAs.

Source

Monoclonal Mouse IgG₁ Clone # 534509

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

NS0 mouse myeloma cell line transfected with human CD157
Gly29-Lys292
Accession # Q10588

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

ELISA

This antibody functions as an ELISA capture antibody when paired with Mouse Anti-Human CD157 Monoclonal Antibody (Catalog # MAB47362).

This product is intended for assay development on various assay platforms requiring antibody pairs.

Flow Cytometry

0.25 µg/10⁶ cells

See below

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

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Detection of CD157 in Human Blood Granulocytes by Flow Cytometry. Human peripheral blood granulocytes were stained with Mouse Anti-Human CD157 Monoclonal Antibody (Catalog # MAB47361, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B).

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CD157

CD157, also known as Bone Marrow Stromal Cell Antigen 1 (BST-1), is a glycosyl phosphatidylinositol anchored membrane protein that belongs to the CD38 family (1). CD157 was discovered in a bone marrow stromal cell line where it facilitates pre-B-cell growth (2, 3). Along with CD38, CD157 is a bifunctional ectoenzyme that exhibits both ADP-ribosyl cyclase and cyclic ADP ribose hydrolase activities (2). It may play a role in rheumatoid arthritis (RA) due to its enhanced expression in
RA-derived bone marrow stromal cell lines (3). CD157 has been predicted to function as a cell surface receptor and an immunoregulatory molecule (4).

References

Hussain, A. M. M. et al. (1998) Protein Express. Purif. 12:133.
Sato, A. et al. (1999) Biochem. J. 337:491.
Kaisho, T. et al. (1994) Proc. Natl. Acad. Sci. USA 91:5325.
Ortolan, E. et al. (2002) Cell Biochem. Funct. 20:309.

Long Name

Cluster of Differentiation 157

Entrez Gene IDs

683 (Human); 12182 (Mouse); 81506 (Rat)

Alternate Names

ADP-ribosyl cyclase 2; Bone marrow stromal antigen 1; bone marrow stromal cell antigen 1; BP-3; BST1; BST-1; cADPr hydrolase 2; CD157 antigen; CD157; Cyclic ADP-ribose hydrolase 2; EC 3.2.2.5; NAD(+) nucleosidase