Human CD74 Antibody Summary
Gln73-Met232
Accession # NP_004346
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of Human CD74 by Western Blot. Western blot shows lysates of Raji human Burkitt's lymphoma cell line and Daudi human Burkitt's lymphoma cell line. PVDF Membrane was probed with 1 µg/mL of Human CD74 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3590) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for CD74 at approximately 35 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
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Detection of Human CD74 by Simple WesternTM. Simple Western lane view shows lysates of Raji human Burkitt's lymphoma cell line and Daudi human Burkitt's lymphoma cell line, loaded at 0.2 mg/mL. Specific bands were detected for CD74 at approximately 49 & 50 kDa (as indicated) using 10 µg/mL of Sheep Anti-Human CD74 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3590) followed by 1:50 dilution of HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: CD74
CD74 is a transmembrane protein which, on antigen presenting cells, is the invariant chain that chaperones MHC class II dimers from the endoplasmic reticulum to the cell surface. The cytoplasmic portion of CD74 can be released by intramembrane cleavage and activate NF kappa B. CD74 is upregulated in several cancers and is also expressed by non-immune cells during inflammation. This antibody was raised to a splice variant of human CD74 which lacks the Thyroglobulin type-1 repeat in the extracellular domain. Within comparable regions, human CD74 shares 70% and 73% amino acid sequence identity with mouse and rat CD74, respectively.
Product Datasheets
Citations for Human CD74 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 3
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Sarcoma Common MHC-I Haplotype Restricts Tumor-Specific CD8+ T Cell Response
Authors: L Mosca, A de Angelis, A Ronchi, A De Chiara, F Fazioli, C Ruosi, L Altucci, M Conte, F de Nigris
Cancers, 2022-07-14;14(14):.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization -
Epithelium- and endothelium-derived exosomes regulate the alveolar macrophages by targeting RGS1 mediated calcium signaling-dependent immune response
Authors: Z Feng, J Zhou, Y Liu, R Xia, Q Li, L Yan, Q Chen, X Chen, Y Jiang, G Chao, M Wang, G Zhou, Y Zhang, Y Wang, H Xia
Cell Death and Differentiation, 2021-03-22;0(0):.
Species: Human
Sample Types: Protein
Applications: ELISA Control -
Efficacy and safety of combined immunosuppressive therapy plus umbilical cord blood infusion in severe aplastic anemia patients: A cohort study
Authors: Xiu Luo, Huina Lu, Bing Xiu, Hao Wu, Bing Li, Ping Li et al.
Experimental and Therapeutic Medicine
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