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Human CD9 Antibody

Catalog #: MAB1880
15 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB1880-SP
MAB1880-100
MAB1880-500
Detection of CD9 in Human Platelets by Flow Cytometry.
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Product Details
Citations (15)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human CD9 Antibody Summary

Species Reactivity
Human
Specificity
Detects human CD9. Stains human CD9 transfectants, but not irrelevant transfectants in flow cytometry.
Source
Monoclonal Mouse IgG2B Clone # 209306
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
NS0 mouse myeloma cell line transfected with human CD9
Met1-Val228
Accession # P21926
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS and NaCl with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Flow Cytometry
0.25 µg/106 cells
See below
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of CD9 antibody in Human Platelets antibody by Flow Cytometry. View Larger

Detection of CD9 in Human Platelets by Flow Cytometry. Human peripheral blood platelets were stained with Mouse Anti-Human CD9 Monoclonal Antibody (Catalog # MAB1880, filled histogram) or isotype control antibody (Catalog # MAB0041, open histogram) followed by anti-Mouse IgG PE-conjugated Secondary Antibody (Catalog # F0102B). View our protocol for Staining Membrane-associated Proteins.

Western Blot Detection of Human CD9 by Western Blot View Larger

Detection of Human CD9 by Western Blot Validation of protein expression by Western blotting.All four exosome samples and their corresponding cell lines were used for validation. Furthermore, supernatant from the pelleted exosomes was used as a control. The selected proteins FASN, XPO1, CD9 and PDCD6IP, were tested with ENO1 and GAPDH as controls. PSA was tested to confirm it is secreted through alternative secretion pathway and therefore not present within exosomes. The nearest protein marker (kDa) is indicated for each blot. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/24391718), licensed under a CC-BY license. Not internally tested by R&D Systems.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CD9

CD9, also known as motility-related protein-1 (MRP-1), is a tetraspan family glycoprotein expressed by a variety of hematopoietic and epithelial cells. It forms homotypic associations as well as heterotypic associations with other tetraspan proteins, some integrins and MHC class II proteins. CD9 has been shown to modulate cellular adhesion, migration and proliferation.

Entrez Gene IDs
928 (Human); 12527 (Mouse)
Alternate Names
BA2; BA-2/p24 antigen; BTCC-1; CD9 antigen; CD9 molecule; CD9; Cell growth-inhibiting gene 2 protein; DRAP-27; Leukocyte antigen MIC3; MIC3; motility related protein-1,5H9 antigen; Motility-related protein; MRP-1FLJ99568; p24; tetraspanin-29; TSPAN29; Tspan-29; TSPAN29MIC3CD9 antigen (p24)

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Citations for Human CD9 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

15 Citations: Showing 1 - 10
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  1. An immunogold single extracellular vesicular RNA and protein (Au SERP) biochip to predict responses to immunotherapy in non-small cell lung cancer patients
    Authors: Luong T. H. Nguyen, Jingjing Zhang, Xilal Y. Rima, Xinyu Wang, Kwang Joo Kwak, Tamio Okimoto et al.
    Journal of Extracellular Vesicles
  2. A Comprehensive Study of Vesicular and Non-Vesicular miRNAs from a Volume of Cerebrospinal Fluid Compatible with Clinical Practice
    Authors: Endika Prieto-Fernández, Ana María Aransay, Félix Royo, Esperanza González, Juan José Lozano, Borja Santos-Zorrozua et al.
    Theranostics
  3. Influence of a pro-inflammatory stimulus on the miRNA and lipid content of human dental stem cell-derived extracellular vesicles and their impact on microglial activation
    Authors: Gratpain, V;Loriot, A;Bottemanne, P;d'Auria, L;Terrasi, R;Payen, VL;van Pesch, V;Muccioli, GG;des Rieux, A;
    Heliyon
    Species: Human
    Sample Types: Protein
    Applications: ELISA Capture
  4. Targeting Antigen to the Surface of EVs Improves the In�Vivo Immunogenicity of Human and Non-human Adenoviral Vaccines in Mice
    Authors: CM Bliss, AJ Parsons, R Nachbagaue, JR Hamilton, F Cappuccini, M Ulaszewska, JP Webber, A Clayton, AVS Hill, L Coughlan
    Mol Ther Methods Clin Dev, 2019-12-24;16(0):108-125.
    Species: Human
    Sample Types: Cell
    Applications: ELISA Capture
  5. Respiratory syncytial virus exhibits differential tropism for distinct human placental cell types with Hofbauer cells acting as a permissive reservoir for infection
    Authors: V Bokun, JJ Moore, R Moore, CC Smallcombe, TJ Harford, F Rezaee, F Esper, G Piedimonte
    PLoS ONE, 2019-12-02;14(12):e0225767.
    Species: Human
    Sample Types: Whole Cells
    Applications: Cell Purification
  6. Neutrophil-derived miR-223 as local biomarker of bacterial peritonitis
    Authors: AC Brook, RH Jenkins, A Clayton, A Kift-Morga, AC Raby, AP Shephard, B Mariotti, SM Cuff, F Bazzoni, T Bowen, DJ Fraser, M Eberl
    Sci Rep, 2019-07-12;9(1):10136.
    Species: Human
    Sample Types: Peritoneal Dialysate
    Applications: ELISA Detection
  7. Transfer of Functional Cargo in Exomeres
    Authors: Q Zhang, JN Higginboth, DK Jeppesen, YP Yang, W Li, ET McKinley, R Graves-Dea, J Ping, CM Britain, KA Dorsett, CL Hartman, DA Ford, RM Allen, KC Vickers, Q Liu, JL Franklin, SL Bellis, RJ Coffey
    Cell Rep, 2019-04-04;0(0):.
    Species: Human
    Sample Types: Exosomes
    Applications: Western Blot
  8. FRZB and melusin, overexpressed in LGMD2A, regulate integrin ?1D isoform replacement altering myoblast fusion and the integrin-signalling pathway
    Authors: O Jaka, L Casas-Frai, M Azpitarte, A Aiastui, A López de M, A Sáenz
    Expert Rev Mol Med, 2017-03-16;19(0):e2.
    Species: Human
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  9. Plasma Exosome Profiling of Cancer Patients by a Next Generation Systems Biology Approach
    Authors: V Domenyuk, Z Zhong, A Stark, N Xiao, HA O'Neill, X Wei, J Wang, TT Tinder, S Tonapi, J Duncan, T Hornung, A Hunter, MR Miglarese, J Schorr, DD Halbert, J Quackenbus, G Poste, DA Berry, G Mayer, M Famulok, D Spetzler
    Sci Rep, 2017-02-20;7(0):42741.
    Species: Human
    Sample Types: Plasma
    Applications: Western Blot
  10. Proteomic profiling of exosomes leads to the identification of novel biomarkers for prostate cancer.
    Authors: Duijvesz D, Burnum-Johnson K, Gritsenko M, Hoogland A, Vredenbregt-van den Berg M, Willemsen R, Luider T, Pasa-Tolic L, Jenster G
    PLoS ONE, 2013-12-31;8(12):e82589.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  11. Focal increases of fetal macrophages in placentas from pregnancies with histological chorioamnionitis: potential role of fibroblast monocyte chemotactic protein-1.
    Authors: Toti P, Arcuri F, Tang Z, Schatz F, Zambrano E, Mor G, Niven-Fairchild T, Abrahams VM, Krikun G, Lockwood CJ, Guller S
    Am. J. Reprod. Immunol., 2010-11-19;65(5):470-9.
    Species: Human
    Sample Types: Whole Cells
    Applications: Cell Selection
  12. Human Embryonic Stem Cells Express Elevated Levels of Multiple Pro-Apoptotic BCL-2 Family Members
    Authors: David T. Madden, Diana Davila-Kruger, Simon Melov, Dale E. Bredesen
    PLoS ONE
  13. Mechanism of Cellular Formation and In Vivo Seeding Effects of Hexameric beta -Amyloid Assemblies
    Authors: Céline Vrancx, Devkee M. Vadukul, Nuria Suelves, Sabrina Contino, Ludovic D’Auria, Florian Perrin et al.
    Molecular Neurobiology
  14. Isolation of villous cytotrophoblasts from second trimester human placentas
    Authors: Tanja Jankovic-Karasoulos, Dale McAninch, Dylan McCullough, Rebecca L. Wilson, Tina Bianco-Miotto, Claire T. Roberts
    Placenta
  15. Stroma‐derived extracellular vesicle mRNA signatures inform histological nature of prostate cancer
    Authors: Alex P. Shephard, Peter Giles, Mariama Mbengue, Amr Alraies, Lisa K. Spary, Howard Kynaston et al.
    Journal of Extracellular Vesicles

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