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Human CD94 Antibody

Catalog #: MAB1058
4 Citations 2 Reviews Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB1058
MAB1058-SP
Product Details
Citations (4)
FAQs
Supplemental Products
Reviews (2)
Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human CD94 Antibody Summary

Species Reactivity
Human
Specificity
Recognizes human CD94 both in its homodimeric form and as a heterodimer with either NKG2A or NKG2C.
Source
Monoclonal Mouse IgG1 Clone # 131412
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
BaF3 mouse pro-B cell line transfected with human CD94 and NKG2A
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Flow Cytometry
0.25 µg/106 cells
Human whole blood CD56+ natural killer cells
Blockade of Receptor-ligand Interaction
HLA-E tetramers at 3 μg/mL were incubated with BaF/3 cells transfected with hNKG2C/CD94, and binding was monitored by flow cytometry. A 15 minute pre-incubation of the transfected cells with this antibody at a concentration of 17 μg/mL inhibited 95% of the HLA-E tetramer binding.
 
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CD94

The type-II transmembrane glycoprotein CD94 covalently associates with C-type lectins of the NKG2 family to yield heterodimers important in NK cell recognition of class I MHC molecules. CD94/NKG2A complexes are also found on a subset of CD8+ T cells. Expression of CD94/NKG2 heterodimers may regulate cell survival and effector functions. There are two alternatively spliced variants of CD94 that differ by 31 amino acids encoded by exon 2.

Entrez Gene IDs
3824 (Human); 16643 (Mouse); 102119829 (Cynomolgus Monkey)
Alternate Names
CD94 antigen; CD94; CD94natural killer cells antigen CD94; Killer cell lectin-like receptor subfamily D member 1; killer cell lectin-like receptor subfamily D, member 1; KLRD1; KP43; NK cell receptor

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Citations for Human CD94 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
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  1. SARS-CoV-2 Nsp13 encodes for an HLA-E-stabilizing peptide that abrogates inhibition of NKG2A-expressing NK cells
    Authors: Q Hammer, J Dunst, W Christ, F Picarazzi, M Wendorff, P Momayyezi, O Huhn, HK Netskar, KT Maleki, M García, T Sekine, E Sohlberg, V Azzimato, M Aouadi, Karolinska, Severe COV, F Degenhardt, A Franke, F Spallotta, M Mori, J Michaëlsso, NK Björkström, T Rückert, C Romagnani, A Horowitz, J Klingström, HG Ljunggren, KJ Malmberg
    Cell Reports, 2022-02-21;0(0):110503.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  2. MicroRNA editing facilitates immune elimination of HCMV infected cells.
    Authors: Nachmani D, Zimmermann A, Oiknine Djian E, Weisblum Y, Livneh Y, Khanh Le V, Galun E, Horejsi V, Isakov O, Shomron N, Wolf D, Hengel H, Mandelboim O
    PLoS Pathog, 2014-02-27;10(2):e1003963.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  3. NKG2A inhibits NKG2C effector functions of gammadelta T cells: implications in health and disease.
    Authors: Angelini DF, Zambello R, Galandrini R, Diamantini A, Placido R, Micucci F, Poccia F, Semenzato G, Borsellino G, Santoni A, Battistini L
    J. Leukoc. Biol., 2010-10-15;89(1):75-84.
    Species: Human
    Sample Types: Whole Cells
    Applications: Functional Assay
  4. CD94 surface density identifies a functional intermediary between the CD56bright and CD56dim human NK-cell subsets.
    Authors: Yu J, Mao HC, Wei M, Hughes T, Zhang J, Park IK, Liu S, McClory S, Marcucci G, Trotta R, Caligiuri MA
    Blood, 2009-11-06;115(2):274-81.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry

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Reviews for Human CD94 Antibody

Average Rating: 2 (Based on 2 Reviews)

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Human CD94 Antibody
By Anonymous on 06/10/2022
Application: Flow Sample Tested: B cell enriched peripheral blood mononuclear cells (PBMC) Species: Human

Red -> HEK cells (neg control)
Blue -> Human PBMCs
***Bio-Techne Response: Thank you for reviewing our product. We are sorry to hear that this product did not perform as expected. We have been in touch with the customer to resolve this issue according to our Product Guarantee and to the customer’s satisfaction.***

Flow Cytometry Human CD94 Antibody MAB1058
Human CD94 Antibody
By Anonymous on 06/10/2022
Application: WB Sample Tested: HEK293T human embryonic kidney cell line Species: Human

1. Mock transfected HEK cells
2. hCD94-transfected HEK cells
***Bio-Techne Response: Thank you for reviewing our product. We are sorry to hear that this product did not perform as expected. We have been in touch with the customer to resolve this issue according to our Product Guarantee and to the customer’s satisfaction.***

Western Blot Human CD94 Antibody MAB1058