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Human CDCP1 Antibody

Catalog #: MAB26661
1 Citation 4 Reviews Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB26661
MAB26661-SP
Product Details
Citations (1)
FAQs
Supplemental Products
Reviews (4)
Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human CDCP1 Antibody Summary

Species Reactivity
Human
Specificity
Detects human CDCP1 in direct ELISAs and Western blots. Recognizes an epitope on human CDCP1 found within the amino acid segment 369‑666.
Source
Monoclonal Mouse IgG2A Clone # 309112
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant human CDCP1 isoform 1
Ala33-Leu666
Accession # NP_073753
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
Recombinant Human CDCP1

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CDCP1

CDCP1, also known as SIMA135, is a novel 140 kDa type I transmembrane glycoprotein with three CUB protein-protein interaction domains in its 635 aa extracellular region. The 148 aa cytoplasmic region contains canonical phosphorylation sites for Src kinase family members and binding sites for SH3 domains. By alternative splicing, a secreted 310 aa residue form of CDCP1 also exists. The amino-terminal region of approximately 265 aa of the type I membrane CDCP1 can also be proteolytically cleaved. CDCP1 is found on the surface of epithelial and bone marrow-derived stem cells. The extracellular region of human CDCP1 shares 84% aa identity with that of the mouse protein.

Long Name
CUB Domain Containing Protein 1
Entrez Gene IDs
64866 (Human); 109332 (Mouse)
Alternate Names
CD318 antigen; CD318; CDCP1; CUB domain containing protein 1; CUB domain-containing protein 1; Membrane glycoprotein gp140; SIMA135; SIMA135TRASK; Subtractive immunization M plus HEp3-associated 135 kDa protein; Transmembrane and associated with src kinases

Product Datasheets

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Citation for Human CDCP1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. The SRC-associated protein CUB Domain-Containing Protein-1 regulates adhesion and motility
    Authors: C H Benes, G Poulogiannis, L C Cantley, S P Soltoff
    Oncogene

FAQs

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Reviews for Human CDCP1 Antibody

Average Rating: 4.3 (Based on 4 Reviews)

5 Star
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Human CDCP1 Antibody
By Anonymous on 01/14/2021
Application: Microarrays Sample Tested: EDTA Plasma Species: Human
Other Human CDCP1 Antibody MAB26661
Human CDCP1 Antibody
By Anonymous on 02/21/2019
Application: MiAr Sample Tested: EDTA Plasma Species: Human
Human CDCP1 Antibody
By Anonymous on 11/08/2018
Application: Luminex Sample Tested: Serum Species: Human
Human CDCP1 Antibody
By Anonymous on 11/08/2018
Application: ELISA Sample Tested: Plasma,Serum Species: Human and Mouse