Human Chk2 Antibody

Name: Human Chk2 Antibody
Brand: R&D Systems
SKU: MAB1358
Rating: 5 (1 reviews)

Catalog #: MAB1358 1 Review Datasheet / COA / SDS

Recombinant Monoclonal Antibody

Catalog #	Availability	Size / Price	Qty
MAB1358-100
MAB1358-SP

Detection of Human Chk2 by Western Blot.

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Chk2 Antibody Summary

Species Reactivity

Human

Specificity

Detects human Chk2 in direct ELISAs.

Source

Recombinant Monoclonal Rabbit IgG Clone # 2521A

Purification

Protein A or G purified from cell culture supernatant

Immunogen

E. coli-derived human Chk2
Asp208-Leu543
Accession # O96017

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human Chk2 by Western Blot. Western blot shows lysates of MDA-MB-468 human breast cancer cell line. PVDF membrane was probed with 1 µg/mL of Rabbit Anti-Human Chk2 Monoclonal Antibody (Catalog # MAB1358) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for Chk2 at approximately 65 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Chk2

Serine/threonine-protein kinase Chk2 (CHEK2) is a 65 kDa member of Serine/threonine-protein kinase family of proteins. Chk2 regulates cell cycle checkpoint arrest through phosphorylation of CDC25A, CDC25B and CDC25C. Inhibition of CDC25 phosphatase activity leads to increased inhibitory tyrosine phosphorylation of CDK-cyclin complexes and blocks cell cycle progression. Chk2 is required for checkpoint-mediated cell cycle arrest, activation of DNA repair and apoptosis in response to the presence of DNA double-strand breaks.Existence of multiple splice variants of Chk2 is predicted. Human Chk2 shares 86% aa sequence identity with mouse Chk2.

Long Name

Checkpoint Kinase 2

Entrez Gene IDs

11200 (Human); 50883 (Mouse); 114212 (Rat)

Alternate Names

CDS1; CHEK2; CHK2 checkpoint homolog (S. pombe); Chk2; EC 2.7.11; EC 2.7.11.1; HuCds1; LFS2; PP1425; Rad53; S.pombe) homolog

Product Datasheets

Product Datasheet

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FAQs

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Reviews for Human Chk2 Antibody

Average Rating: 5 (Based on 1 Review)

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Human Chk2 Antibody

By Anonymous on 03/02/2023

Application: WB Sample Tested: HepG2 human hepatocellular carcinoma cell line Species: Human

Concentration used 0.5 ug/mL

Western Blot Human Chk2 Antibody MAB1358