Human CIB1 Antibody

Catalog #: MAB7557
1 Citation Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB7557
MAB7557-SP

Detection of Human CIB1 by Western Blot.

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All CIB1 Antibodies

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Citations (1)

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human CIB1 Antibody Summary

Species Reactivity

Human

Specificity

Detects human CIB1 in direct ELISAs and Western blots.

Source

Monoclonal Mouse IgG_2B Clone # 791119

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived recombinant human CIB1
Gly2-Leu191
Accession # E9Q3T6

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

2 µg/mL

See below

Immunohistochemistry

8-25 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human CIB1 by Western Blot. Western blot shows lysates of human kidney tissue. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human CIB1 Monoclonal Antibody (Catalog # MAB7557) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for CIB1 at approximately 22 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunohistochemistry

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CIB1 in Human Breast Cancer Tissue. CIB1 was detected in immersion fixed paraffin-embedded sections of human breast cancer tissue using Mouse Anti-Human CIB1 Monoclonal Antibody (Catalog # MAB7557) at 15 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm of cancer cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CIB1

Calcium and Integrin binding protein 1 (CIB1), also known as Calmyrin, is a ubiquitously expressed 22 kDa myristoylated and membrane-associated protein. It contains two EF hand domains (aa 103-138 and aa 148-183). Calmyrin interacts with a range of proteins including Integrin alpha 2b and GPIIb/IIIa, Presenilin-2, InsP3R, Stathmin-2, Myosin 1C, Sphingosine Kinase 1, Polo-like kinases, and Rac3. Human Calmyrin shares 94% aa sequence identity with mouse and rat Calmyrin.

Long Name

Calcium and Integrin Binding 1

Entrez Gene IDs

10519 (Human); 23991 (Mouse); 81823 (Rat)

Alternate Names

calcium and integrin binding 1 (calmyrin); Calcium- and integrin-binding protein; Calmyrin; CIB1; CIBcalcium and integrin binding protein; CIBP; DNA-dependent protein kinase interacting protein; DNA-PKcs-interacting protein; Kinase-interacting protein; KIP; KIP1; KIPcalcium and integrin binding, protein kinase interacting protein; PRKDCIP; SIP2-28; SIP2-28calcium and integrin-binding protein 1; Snk interacting protein 2-28; SNK-interacting protein 2-28

Product Datasheets

Product Datasheet

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Related Research Areas

Citation for Human CIB1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1