Human CIS-1 Antibody Summary
Leu11-Leu258
Accession # Q9NSE2
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of Human CIS-1 by Western Blot. Western blot shows lysates of Nalm-6 human Pre-B acute lymphocytic leukemia cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human CIS-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3194) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for CIS-1 at approximately 35-40 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
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CIS‑1 in HDLM‑2 Human Cell Line. CIS‑1 was detected in immersion fixed HDLM‑2 human Hodgkin’s lymphoma cell line using Goat Anti-Human CIS‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3194) at 15 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. Staining was performed using our protocol for Fluorescent ICC Staining of Non-adherent Cells.
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Detection of Human CIS‑1 by Simple WesternTM. Simple Western lane view shows lysates of NK human natural killer lymphoma cell line, loaded at 0.2 mg/mL. Specific bands were detected for CIS‑1 at approximately 37 and 42 kDa (as indicated) using 10 µg/mL of Goat Anti-Human CIS‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3194). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: CIS-1
Cytokine Inducible SH2-containing protein (CIS-1) is a 29 kDa protein found in a variety of cell types. Mono or polyubiquitination generally results in a 37 or 45 kDa molecule. CIS-1 binds to phosphorylated cytokine receptors IL-3 R beta and EPO-R and blocks downstream activation of STAT5 via receptor internalization and ubiquitin‑mediated proteosomal degradation. Human CIS-1 is a 258 aa peptide that contains one SH2 domain (aa 82‑163) and one SOCS box (aa 218‑258). There are two known alternatively spliced variants with a 7- or 13-aa substitution for the 7 N-terminal amino acid residues. Over the region used as immunogen, human CIS-1 is 91% identical to the corresponding mouse and canine protein sequences.
Product Datasheets
Citations for Human CIS-1 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 2
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The CAGE–MiR-181b-5p–S1PR1 Axis Regulates Anticancer Drug Resistance and Autophagy in Gastric Cancer Cells
Authors: Minjeong Yeon, Youngmi Kim, Deepak Pathak, Eunju Kwon, Dong Young Kim, Myeong Seon Jeong et al.
Frontiers in Cell and Developmental Biology
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Cancer/testis antigen CAGE mediates osimertinib resistance in non-small cell lung cancer cells and predicts poor prognosis in patients with pulmonary adenocarcinoma
Authors: Yeon, M;Lee, H;Yeo, J;Jeong, MS;Jung, HS;Lee, H;Shim, K;Jo, H;Jeon, D;Koh, J;Jeoung, D;
Scientific reports
Species: Human
Sample Types: Tissue Homogenates
Applications: Western Blot
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