Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Western Blot, Immunocytochemistry, Simple Western

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all CIS-1 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human CIS-1
Leu11-Leu258
Accession # Q9NSE2

Specificity

Detects human CIS-1 in direct ELISAs and Western blots.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human CIS‑1 Antibody

Detection of Human CIS-1 antibody by Western Blot.

Detection of Human CIS-1 by Western Blot.

Western blot shows lysates of Nalm-6 human Pre-B acute lymphocytic leukemia cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human CIS-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3194) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for CIS-1 at approximately 35-40 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

CIS‑1 in HDLM‑2 Human Cell Line.

CIS‑1 was detected in immersion fixed HDLM‑2 human Hodgkin’s lymphoma cell line using Goat Anti-Human CIS‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3194) at 15 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. Staining was performed using our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Detection of Human CIS‑1 by Simple WesternTM.

Simple Western lane view shows lysates of NK human natural killer lymphoma cell line, loaded at 0.2 mg/mL. Specific bands were detected for CIS‑1 at approximately 37 and 42 kDa (as indicated) using 10 µg/mL of Goat Anti-Human CIS‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3194). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Applications for Human CIS‑1 Antibody

Application
Recommended Usage

Immunocytochemistry

5-15 µg/mL
Sample: Immersion fixed HDLM‑2 human Hodgkin's lymphoma cell line

Simple Western

10 µg/mL
Sample: NK human natural killer lymphoma cell line

Western Blot

1 µg/mL
Sample: Nalm‑6 human Pre‑B acute lymphocytic leukemia cell line

Reviewed Applications

Read 1 review rated 1 using AF3194 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: CIS-1

Cytokine Inducible SH2-containing protein (CIS-1) is a 29 kDa protein found in a variety of cell types. Mono or polyubiquitination generally results in a 37 or 45 kDa molecule. CIS-1 binds to phosphorylated cytokine receptors IL-3 R beta and EPO-R and blocks downstream activation of STAT5 via receptor internalization and ubiquitin‑mediated proteosomal degradation. Human CIS-1 is a 258 aa peptide that contains one SH2 domain (aa 82‑163) and one SOCS box (aa 218‑258). There are two known alternatively spliced variants with a 7- or 13-aa substitution for the 7 N-terminal amino acid residues. Over the region used as immunogen, human CIS-1 is 91% identical to the corresponding mouse and canine protein sequences.

Long Name

Cytokine Inducible SH2-containing Protein

Alternate Names

CISH

Entrez Gene IDs

1154 (Human); 12700 (Mouse); 83681 (Rat)

Gene Symbol

CISH

UniProt

Q9NSE2

Additional CIS-1 Products

Product Documents for Human CIS‑1 Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human CIS‑1 Antibody

For research use only

Citations for Human CIS‑1 Antibody

Customer Reviews for Human CIS‑1 Antibody (1)

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  • Human CIS-1 Antibody
    Name: Maud TUSSEAU
    Application: Simple Western
    Sample Tested: Hek 293T cell lysate
    Species: Human
    Verified Customer | Posted 08/06/2021
    HEK937T transfected with SOCS1 WT lysates 4 types of lysis : NP40/LAEMMLI/CHAPS/TRITON
    Human CIS‑1 Antibody AF3194
    Bio-Techne Response
    This review was submitted through the legacy Novus Innovators Program, reflecting a new species or application tested on a primary antibody.

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Protocols

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