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Human Claudin-4 Fluorescein-conjugated Antibody

Catalog #: FAB4219F
1 Citation Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
FAB4219F
Detection of Claudin‑4 in PC‑3 Human Cell Line by Flow Cytometry.
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Citations (1)
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Human Claudin-4 Fluorescein-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Detects human Claudin‑4. Stains human Claudin-4 transfectants but not irrelevant transfectants. Recognizes an epitope in the first extracellular loop (aa Met29-Arg81).
Source
Monoclonal Mouse IgG2A Clone # 382321
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
NS0 mouse myeloma cell line transfected with human Claudin-4
Met1-Val209
Accession # NP_001296
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Fluorescein (Excitation= 488 nm, Emission= 515-545 nm)

Applications

Recommended Concentration
Sample
Flow Cytometry
10 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of Claudin‑4 antibody in PC‑3 Human Cell Line antibody by Flow Cytometry. View Larger

Detection of Claudin‑4 in PC‑3 Human Cell Line by Flow Cytometry. PC-3 human prostate cancer cell line was stained with Mouse Anti-Human Claudin-4 Fluorescein-conjugated Monoclonal Antibody (Catalog # FAB4219F, filled histogram) or isotype control antibody (Catalog # IC003F, open histogram). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: Claudin-4

Claudin-4 is a multipass membrane protein in the claudin family of tight junction proteins. Claudin-4 is a 209 amino acid molecule with four transmembrane segments. It is preferentially expressed in intestinal mucosa epithelium and is involved in epithelial tissue development. It is also up-regulated in several carcinomas. Phosphorylation of Claudin-4 regulates paracellular epithelial permeability. Human Claudin-4 shares 83% aa sequence identity with mouse and rat Claudin-4.

Entrez Gene IDs
1364 (Human); 12740 (Mouse); 304407 (Rat)
Alternate Names
claudin 4; Claudin4; Claudin-4; CLDN4; Clostridium perfringens enterotoxin receptor 1; Clostridium perfringens enterotoxin receptor; CPE-R; CPE-RCPETR; CPE-receptor; CPETR1; hCPE-R; WBSCR8; WBSCR8CPERCPETR1claudin-4; Williams-Beuren syndrome chromosomal region 8 protein

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Citation for Human Claudin-4 Fluorescein-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Claudin 4-targeted nanographene phototherapy using a Clostridium perfringens enterotoxin peptide-photosensitizer conjugate
    Authors: G Shim, MG Kim, H Jin, J Kim, YK Oh
    Acta Pharmacol. Sin., 2017-05-29;38(6):954-962.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry

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