Human Claudin-4 Antibody

Catalog #: MAB4219
2 Citations Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB4219
MAB4219-SP

Detection of Claudin‑4 in PC‑3 Human Cell Line by Flow Cytometry.

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All Claudin-4 Antibodies

Product Details

Citations (2)

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Claudin-4 Antibody Summary

Species Reactivity

Human

Specificity

Detects human Claudin‑4. Stains human Claudin-4 transfectants but not irrelevant transfectants. Recognizes an epitope in the first extracellular loop (aa Met29-Arg81).

Source

Monoclonal Mouse IgG_2A Clone # 382321

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

NS0 mouse myeloma cell line transfected with human Claudin-4
Met1-Val209
Accession # NP_001296

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Flow Cytometry

0.25 µg/10⁶ cells

See below

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

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Detection of Claudin‑4 in PC‑3 Human Cell Line by Flow Cytometry. PC-3 human prostate cancer cell line was stained with Mouse Anti-Human Claudin-4 Monoclonal Antibody (Catalog # MAB4219, filled histogram) or isotype control antibody (Catalog # MAB003, open histogram) followed by PE-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Claudin-4

Claudin-4 is a multipass membrane protein in the claudin family of tight junction proteins. Claudin-4 is a 209 amino acid molecule with four transmembrane segments. It is preferentially expressed in intestinal mucosa epithelium and is involved in epithelial tissue development. It is also up‑regulated in several carcinomas. Phosphorylation of Claudin-4 regulates paracellular epithelial permeability. Human Claudin-4 shares 83% aa sequence identity with mouse and rat Claudin-4.

Entrez Gene IDs

1364 (Human); 12740 (Mouse); 304407 (Rat)

Alternate Names

claudin 4; Claudin4; Claudin-4; CLDN4; Clostridium perfringens enterotoxin receptor 1; Clostridium perfringens enterotoxin receptor; CPE-R; CPE-RCPETR; CPE-receptor; CPETR1; hCPE-R; WBSCR8; WBSCR8CPERCPETR1claudin-4; Williams-Beuren syndrome chromosomal region 8 protein

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citations for Human Claudin-4 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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Imaging of Claudin-4 in Pancreatic Ductal Adenocarcinoma Using a Radiolabelled Anti-Claudin-4 Monoclonal Antibody
Authors: Julia Baguña Torres, James C. Knight, Michael J. Mosley, Veerle Kersemans, Sofia Koustoulidou, Danny Allen et al.
Molecular Imaging and Biology
Differentiation-state plasticity is a targetable resistance mechanism in basal-like breast cancer
Authors: T Risom, EM Langer, MP Chapman, J Rantala, AJ Fields, C Boniface, MJ Alvarez, ND Kendsersky, CR Pelz, K Johnson-Ca, LE Dobrolecki, K Chin, AJ Aswani, NJ Wang, A Califano, MT Lewis, CJ Tomlin, PT Spellman, A Adey, JW Gray, RC Sears
Nat Commun, 2018-09-19;9(1):3815.
Species: Human
Sample Types: Cell Lysates
Applications: CyTof