Human Claudin-6 Antibody Summary
Met1-Val220
Accession # P56747
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Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of Claudin-6 in HEK293 Human Cell Line Transfected with Human Claudin-6 and eGFP by Flow Cytometry. HEK293 human cell line transfected with (A) Human Claudin-6 or (B) irrelevant protein, and eGFP was stained with Mouse Anti-Human Claudin-6 Monoclonal Antibody (Catalog # MAB3656) followed by APC-conjugated Goat anti-Mouse IgG Secondary Antibody (Catalog # F0101B). Quadrant markers were set based on Mouse IgG2B Isotype Control Antibody (Catalog # MAB0041). View our protocol for Staining Membrane-associated Proteins.
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Claudin‑6 in iPS2 Human Induced Pluripotent Stem Cells. Claudin-6 was detected in immersion fixed iPS2 human induced pluripotent stem cells, differentiated into definitive endoderm with StemXVivo Endoderm Kit (Catalog # SC019), using Mouse Anti-Human Claudin-6 Monoclonal Antibody (Catalog # MAB3656) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). SOX17 was also detected using Goat Anti-Human SOX17 Antigen Affinity-purified Polyclonal Antibody (green; Catalog # AF1924). Specific staining of Claudin-6 was localized to cell surfaces. View our protocol for Fluorescent ICC Staining of Stem Cells on Coverslips.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Claudin-6
Claudin-6 is a multipass transmembrane protein in the Claudin family. Claudin-6 is expressed by epithelial cells where it participates in tissue development and the maintenance of tight junction integrity. Human Claudin-6 shares 88% and 86% amino acid sequence identity with mouse and rat Claudin-6, respectively.
Product Datasheets
Citations for Human Claudin-6 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 4
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Antibody specificity against highly conserved membrane protein Claudin 6 driven by single atomic contact point
Authors: Brad Screnci, Lewis J. Stafford, Trevor Barnes, Kristen Shema, Samantha Gilman, Rebecca Wright et al.
iScience
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Identification and functional characterization of potential oncofetal targets in human hepatocellular carcinoma
Authors: Mei-Mei Li, Fan-En Kong, Guang-Meng Li, Yi-Ti He, Xiao-Feng Zhang, Cheng-Yang Zhang et al.
STAR Protocols
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A single mutation in the E2 glycoprotein of hepatitis C virus broadens the claudin specificity for its infection
Authors: Y Shirasago, H Fukazawa, S Nagase, Y Shimizu, T Mizukami, T Wakita, T Suzuki, H Tani, M Kondoh, T Kuroda, S Yasuda, Y Sato, K Hanada, M Fukasawa
Scientific Reports, 2022-11-24;12(1):20243.
Species: Human
Sample Types:
Applications: Bioassay -
Identification of novel functions for hepatitis C virus envelope glycoprotein E1 in virus entry and assembly
Authors: J Haddad, Y Rouillé, X Hanoulle, V Descamps, M Hamze, F Dabboussi, TF Baumert, G Duverlie, M Lavie, J Dubuisson
J. Virol, 2017-03-29;0(0):.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry, Functional Assay
FAQs
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Reconstitution Buffers
Secondary Antibodies
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