Human COCO Antibody

Catalog #: MAB3047 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB3047
MAB3047-SP

All COCO Antibodies

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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human COCO Antibody Summary

Species Reactivity

Human

Specificity

Detects human COCO in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant mouse (rm) COCO, rmGremlin, rmPRDC, recombinant human (rh) DAN, rhCerberus, or recombinant chicken Caronte is observed.

Source

Monoclonal Mouse IgG₁ Clone # 322206

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived recombinant human COCO
Arg23-Ala189
Accession # Q8N907

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

Recombinant Human COCO (Catalog # 3047-CC) under non-reducing conditions only

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: COCO

COCO, also known as DAND5, Dante, and CKTSF1B3, is a member of the DAN Domain family of BMP antagonists that includes DAN (DAND1), Gremlin/Drm (DAND2), PRDC (Protein Related to Dan and Cerberus; DAND3), and Cerberus (DAND4). DAN family members contain a cysteine-knot domain that is homologous to that found in other TGF-beta superfamily ligands (1‑3). BMPs play important roles in tissue morphogenesis and development processes (4, 5, 6). The human COCO cDNA encodes a 189 amino acid (aa) precursor with a 22 aa signal sequence (2, 7). COCO has eight Cys residues in the cysteine-knot which places it in the CAN subfamily of BMP antagonists along with the other DAN family proteins (1). Human COCO shares 60% and 24% aa sequence identity with mouse and Xenopus COCO, respectively. It shares 17%, 20%, 25%, and 22% aa sequence identity with human DAN, Gremlin, PRDC, and Cerberus, respectively. In Xenopus embryonal development, COCO is expressed by pluripotent ectodermal cells. Expression is abruptly downregulated prior to gastrulation, and the loss of ectodermal cell pluripotency is coincident with COCO downregulation (7). COCO binds and inhibits Xnr1, BMP-4, Activin, and Wnt-8 (7). In mouse, COCO expression is elevated on the right side of Henson’s node at the early somite stage, in contrast to the left side expression of Nodal (8). COCO may cooperate with Nodal in gastrulation and embryonic left-right axis formation (5, 8).

References

Avsian-Kretchmer, O. and A.J.W. Hsueh (2004) Mol. Endocrinol. 18:1.
Katoh, M. and M. Katoh (2004) Oncol. Rep. 12:423.
Katoh, M. and M. Katoh (2005) Int. J. Mol. Med. 15:885.
Baron, M.H. (2005) Exp. Hematol. 33:1015.
Kishigami, S. and Y. Mishina (2005) Cytokine Growth Factor Rev. 16:265.
De Robertis, E.M. and H. Koruda (2004) Annu. Rev. Cell Dev. Biol. 20:285.
Bell, E. et al. (2003) Development 130:1381.
Pearce, J.J. et al. (1999) Dev. Biol. 209:98.

Entrez Gene IDs

199699 (Human); 23863 (Mouse); 685719 (Rat)

Alternate Names

CER2CRL2; Cerberus 2; Cerberus like-2; cerberus-like 2; Cerberus-like protein 2; CERL2; cerl-2; CKTSF1B3; CKTSF1B3MGC126849; COCO; Cysteine knot superfamily 1, BMP antagonist 3; DAN domain family member 5; DAN domain family, member 5; DAND5; Dante; DTE; FLJ38607; GREM3; GREM3cerberus 2; gremlin-3; SP1