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Human CRISP-3 Antibody

Catalog #: MAB23971
2 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB23971
MAB23971-SP
CRISP‑3 in Human Prostate.
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Citations (2)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human CRISP-3 Antibody Summary

Species Reactivity
Human
Specificity
Detects human CRISP-3 in direct ELISAs and Western blots. In direct ELISAs and Western blots, 50‑100% cross-reactivity with recombinant human CRISP-2 is observed.
Source
Monoclonal Mouse IgG1 Clone # 295203
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant human CRISP-3
Asn21-Tyr245 (Ser106Pro & Ala134Ser)
Accession # P54108.1
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Immunohistochemistry
8-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunohistochemistry CRISP‑3 antibody in Human Prostate by Immunohistochemistry (IHC-P). View Larger

CRISP‑3 in Human Prostate. CRISP-3 was detected in immersion fixed paraffin-embedded sections of human prostate using Mouse Anti-Human CRISP-3 Monoclonal Antibody (Catalog # MAB23971) at 15 µg/mL overnight at 4 °C. Before incubation with the primary antibody tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CRISP-3

CRISP-3 is one of three CRISPs (cysteine-rich secretory proteins) found in mammalian exocrine secretions and granulocytes that may play a role in innate immunity (1‑3). CRISPs and several snake, insect, and lizard venom proteins are characterized by 16 invariant cysteine residues (4). Structurally, they consist of an N-terminal SCP domain, a hinge region, and a cysteine-rich domain (5). CRISP-3 is produced by salivary, pancreas, prostate, and lacrimal glands, as well as spermatozoa and mature spermatids (2, 6, 7). In mouse, however, CRISP-3 has not been detected in the male genital tract (8, 9). CRISP-3 is up-regulated in epithelial prostate cancer and chronic pancreatitis (10, 11). It is present as 30 kDa and 28 kDa species, corresponding to glycosylated and nonglycosylated forms (1, 3, 7, 10, 12). In serum and seminal fluid, CRISP-3 forms high affinity noncovalent complexes with the more abundant alpha 1B-glycoprotein and beta -microseminoprotein/PSP94, respectively (12, 13). Binding is mediated by the SCP domain of CRISP-3 and is independent of glycosylation (12). CRISP-3 is also expressed in pre-B cells but not in T cells or monocytes (14, 15). CRISP-3 is released from neutrophil and eosinophil granules following cell stimulation (1, 15). Mature human CRISP-3 shares 48% and 65% amino acid (aa) sequence identity with mouse and equine CRISP-3, respectively. It shares 44% and 72% aa sequence identity with human CRISP-1 and -2, respectively.

References
  1. Kjeldsen, L. et al. (1996) FEBS Lett. 380:246.
  2. Kratzschmar, J. et al. (1996) Eur. J. Biochem. 236:827.
  3. Udby, L. et al. (2002) J. Immunol. Meth. 263:43.
  4. Yamazaki, Y. and Morita, T. (2004) Toxicon 44:227.
  5. Guo, M. et al. (2005) J. Biol. Chem. 280:12405.
  6. Haendler, B. et al. (1999) J. Cell. Physiol. 178:371.
  7. Udby, L. et al. (2005) J. Androl. 26:333.
  8. Haendler, B. et al. (1993) Endocrinology 133:192.
  9. Haendler, B. et al. (1997) Eur. J. Biochem. 250:440.
  10. Bjartell, A. et al. (2006) Prostate 66:591.
  11. Liao, Q. et al. (2003) Histol. Histopathol. 18:245.
  12. Udby, L. et al. (2005) Biochem. Biophys. Res. Commun. 333:555.
  13. Udby, L. et al. (2004) Biochemistry 43:12877.
  14. Pfisterer, P. et al. (1996) Mol. Cell. Biol. 16:6160.
  15. Udby, L. et al. (2002) J. Leukoc. Biol. 72:462.
Long Name
Cysteine-rich Secretory Protein 3
Entrez Gene IDs
10321 (Human); 11572 (Mouse)
Alternate Names
Aeg2; CRISP3; CRISP-3; CRISP-3MGC126588; CRS3; cysteine-rich secretory protein 3; cysteine-rich secretory protein-3; dJ442L6.3; SGP28; SGP28Aeg2; specific granule protein (28 kDa); Specific granule protein of 28 kDa

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Citations for Human CRISP-3 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. Eukaryotic expression, purification and structure/function analysis of native, recombinant CRISP3 from human and mouse.
    Authors: Volpert, Marianna, Mangum, Jonathan, Jamsai, Duangpor, D'Sylva, Rebecca, O'Bryan, Moira K, McIntyre, Peter
    Sci Rep, 2014-02-27;4(0):4217.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: Western Blot
  2. Expression microarray analysis identifies novel epithelial-derived protein markers in eosinophilic esophagitis.
    Authors: Matoso, Andres, Mukkada, Vincent, Lu, Shaolei, Monahan, Renee, Cleveland, Kelly, Noble, Lelia, Mangray, Shamlal, Resnick, Murray B
    Mod Pathol, 2013-03-15;26(5):665-76.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC

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