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Human CXCR5 Antibody

Catalog #: MAB190
39 Citations 2 Reviews Datasheet / COA / SDS
Clone 51505 was used by HLDA to establish CD designation
Catalog # Availability Size / Price Qty
MAB190-100
MAB190-SP
Detection of CXCR5 in CD19+ Human PBMCs by Flow Cytometry.
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Product Details
Citations (39)
FAQs
Supplemental Products
Reviews (2)
Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human CXCR5 Antibody Summary

Species Reactivity
Human
Specificity
Stains human CXCR5 transfectants but not the parental cell lines in flow cytometry. Does not cross-react with human CXCR2, CXCR3, or CXCR4 transfectants.
Source
Monoclonal Mouse IgG2B Clone # 51505
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
NS0 mouse myeloma cell line transfected with human CXCR5
Met1-Phe372
Accession # P32302
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Flow Cytometry
0.25 µg/106 cells
See below
Immunohistochemistry
8-25 µg/mL
See below
CyTOF-reported
Ferrell, P.B., Jr. et al. (2016) PLoS ONE 11: e0153207. Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 
Immunocytochemistry
8-25 µg/mL
See below
Neutralization
Measured by its ability to neutralize CXCL13/BLC/BCA‑1-induced chemotaxis in the BaF3 mouse pro‑B cell line transfected with human CXCR5. The Neutralization Dose (ND50) is typically 0.25-1.5 µg/mL in the presence of 0.05 µg/mL Recombinant Human CXCL13/BLC/BCA‑1.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of CXCR5 antibody in CD19+ Human PBMCs antibody by Flow Cytometry. View Larger

Detection of CXCR5 in CD19+ Human PBMCs by Flow Cytometry. Human peripheral blood mononuclear cells (PBMCs) were stained with Mouse Anti-Human CD19 APC-conjugated Monoclonal Antibody (Catalog # FAB4867A) and either (A) Mouse Anti-Human CXCR5 Monoclonal Antibody (Catalog # MAB190) or (B) Mouse IgG2B control antibody (Catalog # MAB0041) followed by anti-Mouse IgG PE-conjugated secondary antibody (Catalog # F0102B). View our protocol for Staining Membrane-associated Proteins.

Immunocytochemistry CXCR5 antibody in Human PBMCs by Immunocytochemistry (ICC). View Larger

CXCR5 in Human PBMCs. CXCR5 was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) using Mouse Anti-Human CXCR5 Monoclonal Antibody (Catalog # MAB190) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 637-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL008) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Immunohistochemistry CXCR5 antibody in Human Kidney by Immunohistochemistry (IHC-P). View Larger

CXCR5 in Human Kidney. CXCR5 was detected in immersion fixed paraffin-embedded sections of human kidney using Mouse Anti-Human CXCR5 Monoclonal Antibody (Catalog # MAB190) at 5 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to cell membranes. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Neutralization Chemotaxis Induced by CXCL13/BLC/BCA‑1 and Neutralization by Human CXCR5 Antibody. View Larger

Chemotaxis Induced by CXCL13/BLC/BCA‑1 and Neutralization by Human CXCR5 Antibody. Recombinant Human CXCL13/BLC/BCA-1 (Catalog # 801-CX) chemoattracts the BaF3 mouse pro-B cell line transfected with human CXCR5 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Human CXCL13/BLC/BCA-1 (0.05 µg/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human CXCR5 Monoclonal Antibody (Catalog # MAB190). The ND50 is typically 0.25-1.5 µg/mL.

Western Blot Detection of Human CXCR5 by Western Blot View Larger

Detection of Human CXCR5 by Western Blot Inverse correlation between p53 and CXCR5 expression and function in MCF-7 cells.(A) p53 knock-down results in an increase in the relative abundance of CXCR5 mRNA. The result of 10 experiments is shown. (B) Changes in p53 and CXCR5 protein levels correlate with the levels of corresponding mRNAs. mRNA levels were measured by RT-PCR in real time. Representative data are shown, the experiment was repeated 2 times for p53 and 3 times for CXCR5. Complete western blots are shown in Supplementary Figure S1. (C) CXCR5 stimulates of c-Jun mRNA expression in MCF-7 cells in p53-dependent manner. Cells were exposed to recombinant CXCL13 for 6 hours prior to RNA isolation. The result of three experiments is shown. *P < 0,01 versus MCF-7. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/25786345), licensed under a CC-BY license. Not internally tested by R&D Systems.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CXCR5

CXCR5, also known as BLR-1, is a 7 transmembrane domain protein expressed on B cells. CXCR5 mediates B cell migration following binding of CXCL13.

Entrez Gene IDs
643 (Human); 12145 (Mouse); 29363 (Rat)
Alternate Names
BLR1; BLR1MDR-15; Burkitt lymphoma receptor 1; Burkitt lymphoma receptor 1, GTP binding protein (chemokine (C-X-C motif)receptor 5); Burkitt lymphoma receptor 1, GTP-binding protein; CD185 antigen; CD185; chemokine (C-X-C motif) receptor 5; C-X-C chemokine receptor type 5; CXCR5; CXC-R5; MDR15CXCR-5; MGC117347; Monocyte-derived receptor 15

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Citations for Human CXCR5 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

39 Citations: Showing 1 - 10
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  1. Comparison of Follicular Helper T-Cell Markers with the Expression of the Follicular Homing Marker CXCR5 in Peripheral T-Cell Lymphomas-A Reappraisal of Follicular Helper T-Cell Lymphomas
    Authors: Krenács, L;Krenács, D;Borbényi, Z;Tóth, E;Nagy, A;Piukovics, K;Bagdi, E;
    International journal of molecular sciences
    Species: Human
    Sample Types: Whole Tissue
    Applications: Immunohistochemistry
  2. The Loss of Bcl-6 Expressing T Follicular Helper Cells and the Absence of Germinal Centers in COVID-19
    Authors: Naoki Kaneko, Hsiao-Hsuan Kuo, Julie Boucau, Jocelyn R. Farmer, Hugues Allard-Chamard, Vinay S. Mahajan et al.
    SSRN Electronic Journal
  3. Extrafollicular IgD−CD27−CXCR5−CD11c− DN3 B cells infiltrate inflamed tissues in autoimmune fibrosis and in severe COVID-19
    Authors: Hugues Allard-Chamard, Naoki Kaneko, Alice Bertocchi, Na Sun, Julie Boucau, Hsiao-Hsuan Kuo et al.
    Cell Reports
  4. Cytotoxic CD8+ T cells may be drivers of tissue destruction in Sj�gren's syndrome
    Authors: N Kaneko, H Chen, CA Perugino, T Maehara, R Munemura, S Yokomizo, J Sameshima, TJ Diefenbach, KR Premo, A Chinju, Y Miyahara, M Sakamoto, M Moriyama, JH Stone, S Nakamura, S Pillai
    Scientific Reports, 2022-09-14;12(1):15427.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  5. Temporal changes in T cell subsets and expansion of cytotoxic CD4+ T cells in the lungs in severe COVID-19
    Authors: Naoki Kaneko, Julie Boucau, Hsiao-Hsuan Kuo, Cory Perugino, Vinay S. Mahajan, Jocelyn R. Farmer et al.
    Clinical Immunology
  6. Deciphering and predicting CD4+ T cell immunodominance of influenza virus hemagglutinin
    Authors: Antonino Cassotta, Philipp Paparoditis, Roger Geiger, Ramgopal R. Mettu, Samuel J. Landry, Alessia Donati et al.
    Journal of Experimental Medicine
  7. Loss of Bcl-6-Expressing T Follicular Helper Cells and Germinal Centers in COVID-19
    Authors: Naoki Kaneko, Hsiao-Hsuan Kuo, Julie Boucau, Jocelyn R. Farmer, Hugues Allard-Chamard, Vinay S. Mahajan et al.
    Cell
  8. Lower peripheral helper T cell levels in the synovium are associated with a better response to anti-TNF therapy in rheumatoid arthritis
    Authors: A Julià, G Ávila, R Celis, R Sanmartí, J Ramírez, S Marsal, JD Cañete
    Arthritis Res. Ther., 2020-08-25;22(1):196.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  9. Cytotoxic CD4+ T lymphocytes may induce endothelial cell apoptosis in systemic sclerosis
    Authors: Takashi Maehara, Naoki Kaneko, Cory A. Perugino, Hamid Mattoo, Jesper Kers,, Hugues Allard-Chamard et al.
    Journal of Clinical Investigation
  10. Evidence for a pathogenic role of extrafollicular, IL-10-producing CCR6+B helper T cells in systemic lupus erythematosus
    Authors: F Facciotti, P Larghi, R Bosotti, C Vasco, N Gagliani, C Cordiglier, S Mazzara, V Ranzani, E Rottoli, S Curti, A Penatti, B Karnani, Y Kobayashi, M Crosti, M Bombaci, JP van Hambur, G Rossetti, R Gualtierot, M Gerosa, S Gatti, S Torretta, L Pignataro, SW Tas, S Abrignani, M Pagani, F Grassi, PL Meroni, RA Flavell, J Geginat
    Proc. Natl. Acad. Sci. U.S.A., 2020-03-17;117(13):7305-7316.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  11. Development and application of a high-content virion display human GPCR array
    Authors: Guan-Da Syu, Shih-Chin Wang, Guangzhong Ma, Shuang Liu, Donna Pearce, Atish Prakash et al.
    Nature Communications
  12. The expansion in lymphoid organs of IL-4+ BATF+ T follicular helper cells is linked to IgG4 class switching in vivo
    Authors: Takashi Maehara, Hamid Mattoo, Vinay S Mahajan, Samuel JH Murphy, Grace J Yuen, Noriko Ishiguro et al.
    Life Science Alliance
  13. Role of CXCL13 and CCL20 in the recruitment of B cells to inflammatory foci in chronic arthritis
    Authors: E Armas-Gonz, MJ Domínguez-, A Díaz-Martí, M Arce-Franc, J Castro-Her, G Danelon, V Hernández-, S Bustabad-R, A Cantabrana, M Uguccioni, F Díaz-Gonzá
    Arthritis Res. Ther., 2018-06-07;20(1):114.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  14. Diminished CXCR5 expression in peripheral blood of patients with Sj�gren's syndrome may relate to both genotype and salivary gland homing
    Authors: LA Aqrawi, M Ivanchenko, A Björk, JI Ramírez Se, J Imgenberg-, M Kvarnström, P Haselmayer, JL Jensen, G Nordmark, K Chemin, K Skarstein, M Wahren-Her
    Clin. Exp. Immunol., 2018-03-24;0(0):.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-P
  15. p53-dependent expression of CXCR5 chemokine receptor in MCF-7 breast cancer cells.
    Authors: Mitkin N, Hook C, Schwartz A, Biswas S, Kochetkov D, Muratova A, Afanasyeva M, Kravchenko J, Bhattacharyya A, Kuprash D
    Sci Rep, 2015-03-19;5(0):9330.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  16. Capture-stabilize approach for membrane protein SPR assays.
    Authors: Chu, Ruiyin, Reczek, David, Brondyk, William
    Sci Rep, 2014-12-08;4(0):7360.
    Species: Human
    Sample Types: Peptide
    Applications: Biacore Binding Assay
  17. The MEC1 and MEC2 lines represent two CLL subclones in different stages of progression towards prolymphocytic leukemia.
    Authors: Rasul E, Salamon D, Nagy N, Leveau B, Banati F, Szenthe K, Koroknai A, Minarovits J, Klein G, Klein E
    PLoS ONE, 2014-08-27;9(8):e106008.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  18. CXCR7 influences the migration of B cells during maturation.
    Authors: Humpert M, Pinto D, Jarrossay D, Thelen M
    Eur J Immunol, 2014-02-19;44(3):694-705.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  19. Characterization of AQX-1125, a small-molecule SHIP1 activator: Part 1. Effects on inflammatory cell activation and chemotaxis in vitro and pharmacokinetic characterization in vivo
    Authors: Grant R Stenton, Lloyd F Mackenzie, Patrick Tam, Jennifer L Cross, Curtis Harwig, Jeffrey Raymond et al.
    British Journal of Pharmacology
  20. Blood CD4+CD45RO+CXCR5+ T cells are decreased but partially functional in signal transducer and activator of transcription 3 deficiency.
    Authors: Mazerolles F, Picard C, Kracker S, Fischer A, Durandy A
    J Allergy Clin Immunol, 2013-02-10;131(4):1146-56, 1156.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  21. IL-15 positively regulates IL-21 production in celiac disease mucosa.
    Authors: Sarra M, Cupi M, Monteleone I, Franze E, Ronchetti G, Di Sabatino A, Gentileschi P, Franceschilli L, Sileri P, Sica G, Del Vecchio Blanco G, Cretella M, Paoluzi O, Corazza G, Pallone F, Monteleone G
    Mucosal Immunol, 2012-07-11;6(2):244-55.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  22. In vitro migration capacity of human adipose tissue-derived mesenchymal stem cells reflects their expression of receptors for chemokines and growth factors
    Authors: Sun Jin Baek, Sung Keun Kang, Jeong Chan Ra
    Experimental and Molecular Medicine
  23. Mouse CCL8, a CCR8 agonist, promotes atopic dermatitis by recruiting IL-5+ T(H)2 cells.
    Authors: Islam SA, Chang DS, Colvin RA
    Nat. Immunol., 2011-01-09;12(2):167-77.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  24. IL8 and CXCL13 are potent chemokines for the recruitment of human neural precursor cells across brain endothelial cells.
    Authors: Weiss N, Deboux C, Chaverot N, Miller F, Baron-Van Evercooren A, Couraud P, Cazaubon S
    J Neuroimmunol, 2010-04-18;223(1):131-4.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  25. Serum CXCL13 positively correlates with prostatic disease, prostate-specific antigen and mediates prostate cancer cell invasion, integrin clustering and cell adhesion.
    Authors: Singh S, Singh R, Sharma PK, Singh UP, Rai SN, Chung LW, Cooper CR, Novakovic KR, Grizzle WE, Lillard JW
    Cancer Lett., 2009-04-17;283(1):29-35.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  26. Chemokine CXCL13 is overexpressed in the tumour tissue and in the peripheral blood of breast cancer patients.
    Authors: Panse J, Friedrichs K, Marx A, Hildebrandt Y, Luetkens T, Bartels K, Horn C, Stahl T, Cao Y, Milde-Langosch K, Niendorf A, Kroger N, Wenzel S, Leuwer R, Bokemeyer C, Hegewisch-Becker S, Atanackovic D
    Br. J. Cancer, 2008-09-16;99(6):930-8.
    Species: Human
    Sample Types: Tissue Homogenates, Whole Tissue
    Applications: IHC-P, Western Blot
  27. Altered expression of the receptor-ligand pair CXCR5/CXCL13 in B cells during chronic HIV-1 infection.
    Authors: Cagigi A, Mowafi F, Phuong Dang LV, Tenner-Racz K, Atlas A, Grutzmeier S, Racz P, Chiodi F, Nilsson A
    Blood, 2008-09-09;112(12):4401-10.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-P
  28. Vitamin A metabolites induce gut-homing FoxP3+ regulatory T cells.
    Authors: Kang SG, Lim HW, Andrisani OM, Broxmeyer HE, Kim CH
    J. Immunol., 2007-09-15;179(6):3724-33.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  29. Overexpression of the CXCR5 chemokine receptor, and its ligand, CXCL13 in B-cell chronic lymphocytic leukemia.
    Authors: Burkle A, Niedermeier M, Schmitt-Graff A, Wierda WG, Keating MJ, Burger JA
    Blood, 2007-07-25;110(9):3316-25.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  30. The in vitro migration capacity of human bone marrow mesenchymal stem cells: comparison of chemokine and growth factor chemotactic activities.
    Authors: Ponte AL, Marais E, Gallay N, Langonne A, Delorme B, Herault O, Charbord P, Domenech J
    Stem Cells, 2007-03-29;25(7):1737-45.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  31. Hyaluronan-based polymer scaffold modulates the expression of inflammatory and degradative factors in mesenchymal stem cells: Involvement of Cd44 and Cd54.
    Authors: Lisignoli G, Cristino S, Piacentini A, Cavallo C, Caplan AI, Facchini A
    J. Cell. Physiol., 2006-05-01;207(2):364-73.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  32. Chemokine receptor expression in rat adjuvant-induced arthritis.
    Authors: Haas CS, Martinez RJ, Attia N, Haines GK, Campbell PL, Koch AE
    Arthritis Rheum., 2005-12-01;52(12):3718-30.
    Species: Rat
    Sample Types: Whole Tissue
    Applications: IHC
  33. Plasmacytoid dendritic cell recruitment by immobilized CXCR3 ligands.
    Authors: Kohrgruber N, Groger M, Meraner P, Kriehuber E, Petzelbauer P, Brandt S, Stingl G, Rot A, Maurer D
    J. Immunol., 2004-12-01;173(11):6592-602.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  34. Regulatory T cells can migrate to follicles upon T cell activation and suppress GC-Th cells and GC-Th cell-driven B cell responses.
    Authors: Lim HW, Hillsamer P, Kim CH
    J. Clin. Invest., 2004-12-01;114(11):1640-9.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  35. Developing neonatal rabbit appendix, a primary lymphoid organ, is seeded by immature blood-borne B cells: evidence for roles for CD62L/PNAd, CCR7/CCL21, alpha4beta1 and LFA-1.
    Authors: Sinha RK, Mage RG
    Dev. Comp. Immunol., 2004-06-01;28(7):829-41.
    Species: Rabbit
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  36. Possible link between unique chemokine and homing receptor expression at diagnosis and relapse location in a patient with childhood T-ALL.
    Authors: Annels NE, Willemze AJ, van der Velden VH, Faaij CM, van Wering E, Sie-Go DM, Egeler RM, van Tol MJ, Révész T
    Blood, 2003-12-04;103(7):2806-8.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  37. Expression of functional CCR and CXCR chemokine receptors in podocytes.
    Authors: Huber TB, Reinhardt HC, Exner M, Burger JA, Kerjaschki D, Saleem MA, Pavenstadt H
    J. Immunol., 2002-06-15;168(12):6244-52.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  38. Unique subpopulations of CD56+ NK and NK-T peripheral blood lymphocytes identified by chemokine receptor expression repertoire.
    Authors: Campbell J, Qin S, Unutmaz D, Soler D, Murphy K, Hodge M, Wu L, Butcher E
    J Immunol, 2001-06-01;166(11):6477-82.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  39. Pathologically expanded peripheral T helper cell subset drives B cells in rheumatoid arthritis
    Authors: DA Rao, MF Gurish, JL Marshall, K Slowikowsk, CY Fonseka, Y Liu, LT Donlin, LA Henderson, K Wei, F Mizoguchi, NC Teslovich, ME Weinblatt, EM Massarotti, JS Coblyn, SM Helfgott, YC Lee, DJ Todd, VP Bykerk, SM Goodman, AB Pernis, LB Ivashkiv, EW Karlson, PA Nigrovic, A Filer, CD Buckley, JA Lederer, S Raychaudhu, MB Brenner
    Nature, 2017-02-01;542(7639):110-114.

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Reviews for Human CXCR5 Antibody

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Human CXCR5 Antibody
By Anonymous on 08/19/2021
Application: Immunocytochemistry/Immunofluorescence Sample Tested: Human retinal pigment epithelium cells Species: Human
Immunocytochemistry/Immunofluorescence Human CXCR5 Antibody MAB190
Human CXCR5 Antibody
By Anonymous on 09/11/2020
Application: IHC Sample Tested: Tonsil tissue Species: Human

retrieval in high pH buffer
Ab was diluted 1:2000(0.25ug/mL)

Immunohistochemistry Human CXCR5 Antibody MAB190