Human Cytokeratin 19 Antibody

Detection of Human Cytokeratin 19 by Western Blot. Western blot shows lysates of HT-29 human colon adenocarcinoma cell line, DU145 human prostate carcinoma cell line, HCT-116 human colorectal carcinoma cell line, and MCF-7 human breast cancer cell line. PVDF membrane was probed with 0.1 µg/mL of Sheep Anti-Human Cytokeratin 19 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3506) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Cytokeratin 19 at approximately 40 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Cytokeratin 19 in MCF‑7 Human Cell Line. Cytokeratin 19 was detected in immersion fixed MCF-7 human breast cancer cell line using Sheep Anti-Human Cytokeratin 19 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3506) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; Catalog # NL010) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Cytokeratin 19 in Human Kidney. Cytokeratin 19 was detected in immersion fixed paraffin-embedded sections of human kidney using Sheep Anti-Human Cytokeratin 19 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3506) at 3 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in convoluted tubules. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Detection of KRT19 in Human MCF-7 cell line by Flow Cytometry. MCF-7 human breast cancer cell line was stained with Sheep Anti-Human KRT19 Affinity Purified Polyclonal Antibody (Catalog # AF3506, filled histogram) or control antibody (Catalog # 5-001-A, open histogram) followed by Anti-Sheep IgG PE-conjugated secondary antibody (Catalog # F0126). To facilitate intracellular staining, cells were fixed and permeabilized with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (Catalog # FC012). View our protocol for Staining Membrane-associated Proteins.

Detection of Human Cytokeratin 19 by Simple Western^TM. Simple Western lane view shows lysates of HT-29 human colon adenocarcinoma cell line, loaded at 0.2 mg/mL. A specific band was detected for Cytokeratin 19 at approximately 50 kDa (as indicated) using 1 µg/mL of Sheep Anti-Human Cytokeratin 19 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3506) followed by 1:50 dilution of HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.