Human DPPA4 Antibody

Detection of DPPA4 in NTera‑2 Human Cell Line by Flow Cytometry. NTera-2 human testicular embryonic carcinoma cell line was stained with Sheep Anti-Human DPPA4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3674, filled histogram) or isotype control antibody (Catalog # 5-001-A, open histogram), followed by NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # NL010). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin.

DPPA4 in Human iPSK3 cells. DPPA4 was detected in immersion fixed human plasmid-derived induced pluripotent stem cells (iPSK3) using Sheep Anti-Human DPPA4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3674) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; Catalog # NL010) and counterstained with DAPI (blue). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Stem Cells on Coverslips.

Detection of Mouse DPPA4 by Immunocytochemistry/Immunofluorescence Identification of USP26 as a negative regulator of pluripotency. a Schematic of experimental strategy for screening essential Usps for the generation of iPSCs. Dox-inducible OSKM-transgenic MEFs were plated, transfected with 48 individual mouse Usp shRNA lentiviral vectors to knockdown Usps, and stained for AP+ iPSC colonies after 12 days of Dox treatment. b Quantification of AP+ colonies after 12 days of OSKM induction in MEFs transduced with shRNA, targeting members of the mouse Usp family, p < 0.001 compared to control shRNA. c Quantification of AP+ colonies after 12 days of OSKM induction in MEFs transduced with mouse Usp26 shRNA, Usp20 shRNA, or control shRNA lentivirus, *p < 0.05 compared to control shRNA, **p < 0.01 compared to control shRNA. d Quantification of AP+ colonies after 12 days of OSKM induction in MEFs transduced with pLtet-O (tetracycline-inducible) mouse Usp26, Usp20, or empty vector (EV) lentivirus, **p < 0.01 compared to EV. e Images of AP staining of iPSC colonies after 12 days’ OSKM induction in MEFs transduced with control shRNA, Usp26 shRNA, EV, or pLtet-O lentivirus overexpressing Usp26. f Bright field (BF) and immunofluorescence microscopic images of Oct4, Nanog, and Dppa4 in Usp26 knockdown iPSCs. iPSC colonies were fixed, blocked, and stained with specific antibodies, followed by goat anti-mouse antibody-conjugated Texas Red. Nuclei were stained with DAPI. Scale bar, 100 µM. The data are presented as means ± SD from three independent experiments. b–d Two-way ANOVA for multiple comparisons Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/28839133), licensed under a CC-BY license. Not internally tested by R&D Systems.