Eps8 (EGF R pathway substrate 8) is a 97 kDa member of the Eps family of proteins. It is ubiquitously expressed and serves as a signaling molecule for multiple RTKs, including FGF R, EGF R and PDGF R. Upon activation, Eps8 promotes endocytosis through Rab5, and Rac activation via IRSp53, Abi-1 and Sos-1. Human Eps8 is 822 amino acids (aa) in length. It contains a PH-like domain (aa 62‑173), an EGF R binding region (aa 296‑362), an SH3 domain that mediates homodimerization (aa 535‑587) and a Sos-1 interaction site (aa 648‑821). There is one potential alternate start site at Met261, and a splice variant that shows a 15 aa substitution for aa 676‑822.
Key Product Details
Species Reactivity
Human
Applications
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
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Product Specifications
Immunogen
E. coli-derived recombinant human Eps8
Met1-Ser177
Accession # Q12929
Met1-Ser177
Accession # Q12929
Specificity
Detects endogenous human Eps8 in Western blots.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Human Eps8 Antibody
Detection of Human Eps8 by Western Blot.
Western blot shows lysates of A431 human epithelial carcinoma cell line, MCF-7 human breast cancer cell line, MDA-MB-468 human breast cancer cell line, and A549 human lung carcinoma cell line. PVDF membrane was probed with 1 µg/mL of Human Eps8 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5529) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for Eps8 at approximately 97 kDa (as indicated). This experiment was conducted using Immunoblot Buffer Group 1.
Applications for Human Eps8 Antibody
Application
Recommended Usage
Western Blot
1 µg/mL
Sample: A431 human epithelial carcinoma cell line, MCF-7 human breast cancer cell line, MDA-MB-468 human breast cancer cell line, and A549 human lung carcinoma cell line
Sample: A431 human epithelial carcinoma cell line, MCF-7 human breast cancer cell line, MDA-MB-468 human breast cancer cell line, and A549 human lung carcinoma cell line
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
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Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Calculators
Background: Eps8
Long Name
Epidermal Growth Factor Receptor Pathway Substrate 8
Alternate Names
epidermal growth factor receptor kinase substrate 8, epidermal growth factor receptor pathway substrate 8
Gene Symbol
EPS8
UniProt
Additional Eps8 Products
Product Documents for Human Eps8 Antibody
Product Specific Notices for Human Eps8 Antibody
For research use only
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Cellular Response to Hypoxia Protocols
- R&D Systems Quality Control Western Blot Protocol
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars