Human ESAM Antibody Summary
Gln30-Ala247
Accession # Q96AP7
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of ESAM in HUVEC Human Cells by Flow Cytometry. HUVEC human umbilical vein endothelial cells were stained with Mouse Anti-Human ESAM Monoclonal Antibody (Catalog # MAB4204, filled histogram) or isotype control antibody (Catalog # MAB0041, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG F(ab')2Secondary Antibody (Catalog # F0102B).
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ESAM in HUVEC Human Cells. ESAM was detected in immersion fixed HUVEC human umbilical vein endothelial cells using Mouse Anti-Human ESAM Monoclonal Antibody (Catalog # MAB4204) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conju-gated Anti-Mouse IgG Secondary Antibody (yellow; Catalog # NL007) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: ESAM
Endothelial cell-selective adhesion molecule (ESAM) is a 55 kDa type I transmembrane glycoprotein that belongs to the JAM family of immunoglobulin superfamily molecules (1, 2). Human ESAM is synthesized as a 390 amino acid (aa) protein composed of a 29 aa signal peptide, a 216 aa extracellular region, a putative 26 aa transmembrane segment, and a 119 aa cytoplasmic domain. The extracellular region contains one V-type and one C2-type Ig domain and is involved in homophilic adhesion (1). In the cytoplasmic domain, there is a docking site for the multifunctional adaptor protein MAGI-1 (3). The extracellular region of human ESAM shows 90%, 74%, 69%, and 67% aa identity with monkey, canine, mouse, and rat extracellular ESAM, respectively. ESAM is expressed on endothelial cells, activated platelets, and megakaryocytes and can be found associated with cell-to-cell junctions. Whether ESAM is restricted to a particular junctional type is not clear (1, 2). ESAM deficient mice have no defect in vascularization but do have reduced angiogenic potential. This may be due to a decreased migratory response to FGF-2 (4).
- Hirata, K-I. et al. (2001) J. Biol. Chem. 276:16223.
- Nasdala, I. et al. (2002) J. Biol. Chem. 277:16294.
- Wegmann, F. et al. (2004) Exp. Cell Res. 300:121.
- Ishida, T. et. al. (2003) J. Biol. Chem. 278:34598.
Product Datasheets
Citations for Human ESAM Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 2
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In situ molecular characterization of endoneurial microvessels that form the blood-nerve barrier in normal human adult peripheral nerves.
Authors: Xuan Ouyang, Chaoling Dong, Eroboghene E. Ubogu
Journal of the Peripheral Nervous System
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The Human Blood-Nerve Barrier Transcriptome
Authors: Steven P. Palladino, E. Scott Helton, Preti Jain, Chaoling Dong, Michael R. Crowley, David K. Crossman et al.
Scientific Reports
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