Home / EVI-1 / Human EVI-1 Antibody

Human EVI-1 Antibody

Catalog #: MAB75061
1 Citation Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB75061
MAB75061-SP
EVI‑1 in SK‑OV‑3 Human Cell Line.
1 Image
Product Details
Citations (1)
FAQs
Supplemental Products
Reviews
Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human EVI-1 Antibody Summary

Species Reactivity
Human
Specificity
Detects human EVI-1 in direct ELISAs.
Source
Monoclonal Mouse IgG2B Clone # 702321
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant human EVI-1
Gly241-Met430
Accession # Q03112
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Immunocytochemistry
8-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunocytochemistry EVI-1 antibody in SK-OV-3 Human Cell Line by Immunocytochemistry (ICC). View Larger

EVI‑1 in SK‑OV‑3 Human Cell Line. EVI-1 was detected in immersion fixed SK-OV-3 human ovarian adenocarcinoma cell line using Mouse Anti-Human EVI-1 Monoclonal Antibody (Catalog # MAB75061) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Preparation and Storage

Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL.
Loading...
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: EVI-1

Ectopic virus integration site 1 (EVI-1), also known as MECOM, is a 145 kDa is a transcriptional regulator that interacts with GATA2 and histone methyltransferases. EVI-1 contains 7 tandem N-terminal zinc finger regions (aa 21-239), a central region, and a cluster of 3 more zinc fingers (aa 733-812). Longer isoforms have 189 aa or 64 aa N-terminal extensions. EVI-1 target genes are critical to hematopoietic stem cell proliferation and myeloid differentiation. EVI-1 is overexpressed in acute myelogenous leukemia (AML) as well as ovarian cancer. Chromosomal translocations fuse EVI-1 with RUNX1 and RPN1 contribute to chromosomal instability, myeloid leukemia proliferation, and a block in myeloid differentiation. Within aa 241-430, human EVI-1 shares 94% aa sequence identity with mouse and rat EVI-1.

Long Name
Ecotropic Viral Integration Site 1
Entrez Gene IDs
2122 (Human); 14013 (Mouse); 294924 (Rat)
Alternate Names
AML1-EVI-1 fusion protein; AML1-EVI-1; ecotropic viral integration site 1; Ecotropic virus integration site 1 protein homolog; EVI1; EVI-1; EVI1MDS1-EVI1; MDS1 and EVI1 complex locus protein EVI1; MDS1 and EVI1 complex locus protein MDS1; MDS1 and EVI1 complex locus; MDS1; MECOM; MGC163392; MGC97004; Myelodysplasia syndrome 1 protein; myelodysplasia syndrome 1; Myelodysplasia syndrome-associated protein 1; oncogene EVI1; PRDM3; zinc finger protein Evi1

Product Datasheets

You must select a language.

x
Product Datasheet
COA
SDS

Citation for Human EVI-1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Progressive Recruitment of Mesenchymal Progenitors Reveals a Time-Dependent Process of Cell Fate Acquisition in Mouse and Human Nephrogenesis
    Authors: Lindstrom NO, De Sena Brandine G, Tran T et al.
    Dev. Cell

FAQs

No product specific FAQs exist for this product, however you may

View all Antibody FAQs
Loading...

Reviews for Human EVI-1 Antibody

There are currently no reviews for this product. Be the first to review Human EVI-1 Antibody and earn rewards!

Have you used Human EVI-1 Antibody?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥2500 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review