Human FATP2 Antibody

Catalog #: MAB4659 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB4659
MAB4659-SP

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Detection of FATP2 in HepG2 Human Cell Line by Flow Cytometry.

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All FATP2 Antibodies

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human FATP2 Antibody Summary

Species Reactivity

Human

Specificity

Detects human FATP2. Stains human FATP2 transfectants but not irrelevant transfectants.

Source

Monoclonal Mouse IgG₁ Clone # 466106

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

HEK293 human embryonic kidney cell line transfected with human FATP2
Met1-Leu620
Accession # O14975

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Intracellular Staining by Flow Cytometry

2.5 µg/10⁶ cells

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Intracellular Staining by Flow Cytometry

Detection of FATP2 antibody in HepG2 Human Cell Line antibody by Flow Cytometry.

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Detection of FATP2 in HepG2 Human Cell Line by Flow Cytometry. HepG2 human hepatocellular carcinoma cell line was stained with Mouse Anti-Human FATP2 Mono-clonal Anti-body (Catalog # MAB4659, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG F(ab')₂Secondary Anti-body (Catalog # F0102B). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Reconstitution Buffer Available

Reconstitution Buffer 1 (PBS)

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: FATP2

Fatty Acid Transport Protein 2 (FATP2), also known as SLC27A2 (solute carrier family 27, member 2), is a multipass transmembrane protein that participates in the transport and metabolism of long chain fatty acids. It has acyl-CoA synthase activity and contains an AMP-binding motif. FATP2 localizes to the endoplasmic reticulum and is predominantly expressed in the liver and kidney. Human FATP2 shares 82% aa sequence identity with mouse and rat FATP2.

Long Name

Fatty Acid Transport Protein 2

Entrez Gene IDs

11001 (Human); 26458 (Mouse); 65192 (Rat)

Alternate Names

ACSVL1; ACSVL1FATP-2; EC 6.2.1; EC 6.2.1.-; EC 6.2.1.3; EC 6.2.1.7; FACVL1; FACVL1very long-chain fatty-acid-coenzyme A ligase 1; FATP2; FATP2THCA-CoA ligase; Fatty acid transport protein 2; Fatty-acid-coenzyme A ligase, very long-chain 1; hFACVL1; HsT17226; Long-chain-fatty-acid--CoA ligase; SLC27A2; solute carrier family 27 (fatty acid transporter), member 2; Solute carrier family 27 member 2; THCA-CoA ligase; very-long-chain acyl-CoA synthetase; VLACS; VLACSVery long-chain-fatty-acid-CoA ligase; VLCS; VLCSvery long-chain acyl-CoA synthetase