Human FCRLA Antibody

Catalog #: AF7965 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF7965
AF7965-SP

Detection of Human FCRLA by Western Blot.

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human FCRLA Antibody Summary

Species Reactivity

Human

Specificity

Detects human FCRLA in direct ELISAs and Western blots. In direct ELISAs, approximately 5-10% cross-reactivity with recombinant mouse FCRLA is observed.

Source

Polyclonal Sheep IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human FCRLA
Pro270-Glu359
Accession # Q7L513

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human FCRLA by Western Blot. Western blot shows lysates of Ramos human Burkitt's lymphoma cell line and Raji human Burkitt's lymphoma cell line. PVDF membrane was probed with 0.1 µg/mL of Sheep Anti-Human FCRLA Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7965) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for FCRLA at approximately 44 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Sterile PBS to a final concentration of 0.2 mg/mL.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: FCRLA

FCRLA (Fc Receptor-Like A; also known as FcRX, FcRL1 and Fc receptor Homolog Expressed in B cells/FREB) is a 40-44 kDa intracellular member of the Fc gamma RI class, FcR family, Immunoglobulin Superfamily of molecules. Initially, it is associated with germinal center B cells that are undergoing somatic hypermutation and class-switch recombination. Now, it is believed that all B cells (but not CD38⁺ plasma cells) are also FCRLA⁺. FCRLA is suggested to act as an ER chaperone during antibody maturation, and is known to bind to IgM, IgA, and IgG prior to their secretion. Human FCRLA is synthesized as a 359 amino acid (aa) precursor that contains a 27 aa signal sequence plus a mature region that contains two C2-type Ig-like domains (aa 70-159 and 170-257), and a C-terminal poly-Proline region (aa 269-315). Although there is no traditional ER retention signal, a viable substitute is assumed to exist in the N-terminus of the mature molecule. FCLRA is believed to exist naturally as a monomer; however, disulfide-linkage can occur during experimental manipulation. At least eight potential isoform variants have been reported. It is unclear if, or how frequently they are expressed. One utilizes an alternative start site 17 aa upstream of the standard site, a second contains a six aa insertion after Ala28, and a third possessess an four aa substitution for aa 29-166. Five show single block deletions; in random order, they involve aa 78-166, 27-166, 78-261, 21‑261 and 167-26. Over aa 270-359, human FCLRA shares 55% aa sequence identity with mouse FCRLA.

Long Name

Fc Receptor-like A

Entrez Gene IDs

84824 (Human); 98752 (Mouse)

Alternate Names

Fc receptor homolog expressed in B cells (FREB); Fc receptor homolog expressed in B-cells; Fc receptor related protein X; Fc receptor-like A; Fc receptor-like and mucin-like 1; Fc receptor-related protein X; FCRL1; FCRLA; FCRLb; FCRLc1; FCRLc2; FCRLd; FCRLe; FCRLFc receptor-like protein; FCRLM1; FCRLX; FcRX; FREB; FREBFc receptor-like and mucin-like protein 1; MGC4595